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 >  Protein>Spike RBD >SPD-C82E8

Biotinylated SARS-CoV-2 (COVID-19) S protein RBD, His,Avitag™ (MALS verified)

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分子别名(Synonym)

Spike,S protein RBD,Spike glycoprotein Receptor-binding domain,S glycoprotein RBD,Spike protein RBD

表达区间及表达系统(Source)

Biotinylated SARS-CoV-2 S protein RBD, His,Avitag (SPD-C82E8) is expressed from human 293 cells (HEK293).

Predicted N-terminus: Arg 319

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)

The protein has a calculated MW of 28.2 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Supplied as 0.2 μm filtered solution in 10 mM PB, pH7.4.

Contact us for customized product form or formulation.

运输(Shipping)

This product is supplied and shipped with dry ice, please inquire the shipping cost.

存储(Storage)

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. The product MUST be stored at -70°C or lower upon receipt;
  2. -70°C for 3 months under sterile conditions.
 

电泳(SDS-PAGE)

Spike RBD SDS-PAGE

Biotinylated SARS-CoV-2 S protein RBD, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Spike RBD SEC-MALS

The purity of Biotinylated SARS-CoV-2 S protein RBD, His,Avitag (Cat. No. SPD-C82E8) is more than 90% and the molecular weight of this protein is around 30-40 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Spike RBD ELISA

Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 2 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 S protein RBD, His,Avitag (Cat. No. SPD-C82E8) with a linear range of 10-313 ng/mL (QC tested).

Protocol

背景(Background)

Its been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

 

文献引用(Citations)

 

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