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 >  Protein>EMMPRIN >CD7-HF222

FITC-Labeled Human EMMPRIN / CD147 Protein, His Tag

分子别名(Synonym)

Basigin,BSG,5F7,CD147,EMMPRIN,M6,OK,TCSF

表达区间及表达系统(Source)

FITC-Labeled Human EMMPRIN, His Tag (CD7-HF222) is expressed from human 293 cells (HEK293). It contains AA Ala 22 - His 205 (Accession # P35613-2). It is the FITC labeled form of Human EMMPRIN, His Tag (CD7-H5222).

Predicted N-terminus: Ala 22

Request for sequence

蛋白结构(Molecular Characterization)

Online(Ala 22 - His 205) P35613-2

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 20.9 kDa. The protein migrates as 28-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Conjugate

FITC

Excitation source: 488 nm spectral line, argon-ion laser

Excitation Wavelength: 488 nm

Emission Wavelength: 535 nm

标记(Labeling)

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.

蛋白标记度(FITC:Protein Ratio)

The FITC to protein molar ratio is 1-3.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

FITC-Labeled Human EMMPRIN, His Tag (Cat. No. CD7-HF222) SDS-PAGE gel

FITC-Labeled Human EMMPRIN, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Anti-EMMPRIN Antibody, Mouse IgG1 at 5 μg/mL (100 μL/well) can bind FITC-Labeled Human EMMPRIN, His Tag (Cat. No. CD7-HF222) with a linear range of 0.01-0.078 μg/mL (QC tested).

Protocol

背景(Background)

CD147 is also known as Basigin (BSG), or extracellular matrix metalloproteinase inducer (EMMPRIN). The human basigin protein contains 269 amino acids that form two heavily glycosylated C2 type immunoglobulin-like domains at the N-terminal extracellular portion. A second form of basigin has also been characterized that contains one additional immunoglobulin-like domain in its extracellular portion. As members of the immunoglobulin superfamily play fundamental roles in intercellular recognition involved in various immunologic phenomena, differentiation, and development, basigin is thought also to play a role in intercellular recognition and regulate several distinct functions, such as spermatogenesis, expression of the monocarboxylate transporter and the responsiveness of lymphocytes. Basigin is a type I integral membrane receptor that has many ligands, including the cyclophilin (CyP) proteins Cyp-A and CyP-B and certain integrins.

 

 

前沿进展

 
 
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EMMPRIN靶点信息
英文全称:Basigin
中文全称:Basigin蛋白
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:5详情
最高研发阶段:批准上市
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