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 >  Protein>CEACAM-5 >CE5-HF254

FITC-Labeled Human CEACAM-5 / CD66e Protein, His Tag (MALS verified)

分子别名(Synonym)

CEACAM-5,CD66e,CEA,Meconium antigen 100

表达区间及表达系统(Source)

FITC-Labeled Human CEACAM-5, His Tag (CE5-HF254) is expressed from human 293 cells (HEK293). It contains AA Lys 35 - Ala 685 (Accession # P06731-1). It is the FITC labeled form of Human CEACAM-5, His Tag (Cat. No.CE5-H5226).

Predicted N-terminus: Lys 35

Request for sequence

蛋白结构(Molecular Characterization)

Online(Lys 35 - Ala 685) P06731-1

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 72.2kDa. The protein migrates as 90-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Conjugate

FITC

Excitation source: 488 nm spectral line, argon-ion laser

Excitation Wavelength: 488 nm

Emission Wavelength: 535 nm

标记(Labeling)

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.

蛋白标记度(FITC:Protein Ratio)

The FITC to protein molar ratio is 1-3.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

FITC-Labeled Human CEACAM-5, His Tag (Cat. No. CE5-HF254) SDS-PAGE gel

FITC-Labeled Human CEACAM-5, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

FITC-Labeled Human CEACAM-5, His Tag (Cat. No. CE5-HF254) MALS images

The purity of FITC-Labeled Human CEACAM-5, His Tag (Cat. No. CE5-HF254) is more than 90% and the molecular weight of this protein is around 160-180 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Anti-CEACAM-5 (labetuzumab) Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind FITC-Labeled Human CEACAM-5, His Tag (Cat. No. CE5-HF254) with a linear range of 0.039-0.625 μg/mL (QC tested).

Protocol

背景(Background)

Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) is also Carcinoembryonic antigen (CEA), Meconium antigen 100, CD antigen CD66e, CEACAM5 belongs to the immunoglobulin superfamily and CEA family. CEACAM5 contains seven Ig-like (immunoglobulin-like) domains. CEACAM5 is homodimer protein which binding of E. coli Dr adhesins leads to dissociation of the homodimer. CEACAM5 is cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. CEACAM5 is receptor for E. coli Dr adhesins.

 

 

前沿进展

 
 
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CEACAM-5靶点信息
英文全称:Carcinoembryonic antigen related cell adhesion molecule 5
中文全称:癌胚抗原相关细胞粘附分子5
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:10详情
最高研发阶段:临床三期
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