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Human Carbonic Anhydrase IX / CA9 (38-414) Protein, His Tag (active enzyme)

分子别名(Synonym)

CAIX,CA9,CA-IX,G250,MN,P54/58N,pMW1

表达区间及表达系统(Source)

Human Carbonic Anhydrase IX (38-414), His Tag (CA9-H5226) is expressed from human 293 cells (HEK293). It contains AA Gln 38 - Asp 414 (Accession # Q16790-1).

Predicted N-terminus: Gln 38

蛋白结构(Molecular Characterization)

Online(Gln 38 - Asp 414) Q16790-1

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 41.7 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 20 mM MES, 100 mM NaCl, pH6.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human Carbonic Anhydrase IX (38-414), His Tag (Cat. No. CA9-H5226) SDS-PAGE gel

Human Carbonic Anhydrase IX (38-414), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)

The specific activity is >200 pmol/min/μg, as measured with 4-Nitrophenyl acetate (Routinely tested). 

One unit is defined as the amount of enzyme that hydrolyze 1.0 p mole of 4-Nitrophenyl acetate to 4-Nitrophenol per minute at pH7.5 at Room temperature

Protocol

 

背景(Background)

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes. CAs form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa), a reversible reaction that occurs rather slowly in the absence of a catalyst. One of the functions of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues. The active site of most carbonic anhydrases contains a zinc ion. There are at least five distinct CA families (α, β, γ, δ and ε). Carbonic anhydrase 9 (CA9 / CAIX) is also known as Membrane antigen MN (MN), Renal cell carcinoma-associated antigen G250, which belongs to the alpha-carbonic anhydrase family. CA9 / CAIX with an optimal activity at pH 6.49. Reversible hydration of carbon dioxide. CA IX participates in pH regulation. CA9 may be involved in the control of cell proliferation and transformation. CA-IX appears to be a novel specific biomarker for a cervical neoplasia.

 

前沿进展

 
货号/价格

CA9-H5226-50ug / ¥3500

CA9-H5226-1mg (200ug X 5) / ¥22000

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Carbonic Anhydrase IX靶点信息
英文全称:Carbonic anhydrase IX
中文全称:碳酸酐酶IX
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:12详情
最高研发阶段:临床二期
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