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Biotinylated Human Syndecan-1 Protein, Fc,Avitag™

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利用生物素-链霉亲和素系统的高特异性和高灵敏度的结合特性,可将生物素化蛋白与链霉亲和素相关产品联合应用于各类实验,与非生物素体系相比,检测速度更快、结合能力更强、非特异背景更低。

分子别名(Synonym)

SDC1,Syndecan-1,CD138,SYND1,SDC

表达区间及表达系统(Source)

Biotinylated Human Syndecan-1, Fc,Avitag (SY1-H82F3) is expressed from human 293 cells (HEK293). It contains AA Gln 23 - Gly 254 (Accession # P18827-1).

Predicted N-terminus: Gln 23

Request for sequence

蛋白结构(Molecular Characterization)

Syndecan-1 Structure

This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 52.0 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to Glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Syndecan-1 SDS-PAGE

Biotinylated Human Syndecan-1, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

Syndecan-1 ELISA

Immobilized Biotinylated Human Syndecan-1, Fc,Avitag (Cat. No. SY1-H82F3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) (0.5 μg/well) plate. can bind Anti-Syndecan-1 antibody, Mouse IgG1 with a linear range of 0.2-6 ng/mL (QC tested).

Protocol

Syndecan-1 ELISA

Immobilized Anti-Syndecan-1 antibody, Mouse IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human Syndecan-1, Fc,Avitag (Cat. No. SY1-H82F3) with a linear range of 0.5-8 ng/mL (Routinely tested).

Protocol

 

活性(Bioactivity)-FACS

Syndecan-1 FACS

2e5 of anti-Syndecan-1 CAR-293 cells were stained with 100 μL of 0.3 μg/mL of Biotinylated Human Syndecan-1, Fc,Avitag (Cat. No. SY1-H82F3) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).

Protocol

 

背景(Background)

Syndecan-1 (SYND1 or SDC1) is also known as CD antigen CD138, is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 / SDC1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a useful marker for plasma cells, but only if the cells tested are already known to be derived from blood.

 

前沿进展

 
 
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Syndecan-1靶点信息
英文全称:Syndecan-1
中文全称:多配体蛋白聚糖-1
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:2详情
最高研发阶段:临床二期
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