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Biotinylated Human BAFFR / TNFRSF13C Protein, His,Avitag™ (MALS verified)

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分子别名(Synonym)

BAFFR,TNFRSF13C,BROMIX,CD268,CVID4,prolixin,BAFF-R

表达区间及表达系统(Source)

Biotinylated Human BAFFR, His,Avitag (BAR-H82E3) is expressed from human 293 cells (HEK293). It contains AA Ser 7 - Ala 71 (Accession # Q96RJ3-1).

Predicted N-terminus: Ser 7

Request for sequence

蛋白结构(Molecular Characterization)

Online(Ser 7 - Ala 71) Q96RJ3-1

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 10.1 kDa. The protein migrates as 15-22 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human BAFFR, His,Avitag (Cat. No. BAR-H82E3) SDS-PAGE gel

Biotinylated Human BAFFR, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

Biotinylated Human BAFFR, His,Avitag (Cat. No. BAR-H82E3) MALS images

The purity of Biotinylated Human BAFFR, His,Avitag (Cat. No. BAR-H82E3) is more than 85% and the molecular weight of this protein is around 12-20 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human BAFF, Fc Tag, active trimer (Cat. No. BAF-H5261) at 1 μg/mL (100 μL/well) can bind Biotinylated Human BAFFR, His,Avitag (Cat. No. BAR-H82E3) with a linear range of 0.2-2 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Anti-BAFFR Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human BAFFR, His,Avitag (Cat. No. BAR-H82E3) with a linear range of 0.2-0.8 ng/mL (Routinely tested).

Protocol

Biotinylated Human ELISA

Immobilized Biotinylated Human BAFFR, His,Avitag (Cat. No. BAR-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-BAFFR Antibody, Human IgG1 with a linear range of 0.2-3 ng/mL (Routinely tested).

Protocol

背景(Background)

BAFF receptor (B-cell activating factor receptor, BAFF-R), also known as tumor necrosis factor receptor superfamily member 13C (TNFRSF13C), is a membrane protein of the TNF receptor superfamily which recognizes BAFF. B-cell activating factor (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. Overexpression of BAFF in mice results in mature B-cell hyperplasia and symptoms of systemic lupus erythematosus (SLE). Also, some SLE patients have increased levels of BAFF in serum. Therefore, it has been proposed that abnormally high levels of BAFF may contribute to the pathogenesis of autoimmune diseases by enhancing the survival of autoreactive B cells.

 

 

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BAFFR靶点信息
英文全称:B-cell activating factor receptor
中文全称:B细胞活化因子受体
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:4详情
最高研发阶段:临床三期
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