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Human Carbonic Anhydrase II / CA2 Protein, His Tag

分子别名(Synonym)

CA2,CA-II,CAII,Car2

表达区间及表达系统(Source)

Human CA2, His Tag (CA2-H5228) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Lys 260 (Accession # NP_000058.1).

Predicted N-terminus: Met 1

蛋白结构(Molecular Characterization)

Online(Met 1 - Lys 260) NP_000058.1

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 30.1 kDa. The protein migrates as 31-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 150 mM NaCl, pH8.0. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human CA2, His Tag (Cat. No. CA2-H5228) SDS-PAGE gel

Human CA2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

文献引用(Citations)

背景(Background)

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes. CAs form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa), a reversible reaction that occurs rather slowly in the absence of a catalyst. One of the functions of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues. The active site of most carbonic anhydrases contains a zinc ion. They are, therefore, classified as metalloenzymes. There are at least five distinct CA families (α, β, γ, δ and ε). These families have no significant amino acid sequence similarity and in most cases are thought to be an example of convergent evolution. The α-CAs are found in humans. Carbonic anhydrase II (CA2) is also known as Carbonate dehydratase II, Carbonic anhydrase C, is one of fourteen forms of human α carbonic anhydrases. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Renal carbonic anhydrase allows the reabsorption of sodium ions in the proximal tubule. Carbonic anhydrase II has been shown to interact with Band 3 and Sodium-hydrogen antiporter 1.

 

前沿进展

 
货号/价格

CA2-H5228-100ug / ¥2500

CA2-H5228-1mg (500ug X 2) / ¥15000

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Carbonic Anhydrase II靶点信息
英文全称:Carbonic anhydrase II
中文全称:碳酸酐酶II
种类:Homo sapiens
上市药物数量:8详情
临床药物数量:3详情
最高研发阶段:批准上市
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