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 >  Protein>CD7 >CD7-HF257

FITC-Labeled Human CD7 Protein, Llama IgG2b Fc Tag

分子别名(Synonym)

CD7,GP40,TP41,LEU-9,Tp40

表达区间及表达系统(Source)

FITC-Labeled Human CD7, Llama IgG2b Fc Tag (CD7-HF257) is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Pro 180 (Accession # P09564-1). It is the FITC labeled form of Human CD7, Llama IgG2b Fc Tag, low endotoxin (CD7-H5258).

Predicted N-terminus: Glu

Request for sequence

蛋白结构(Molecular Characterization)

CD7 Structure

This protein carries a llama IgG2b Fc tag at the N-terminus.

The protein has a calculated MW of 44.3 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

偶联(Conjugate)

FITC

Excitation source: 488 nm spectral line, argon-ion laser

Excitation Wavelength: 488 nm

Emission Wavelength: 535 nm

标记(Labeling)

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

CD7 SDS-PAGE

FITC-Labeled Human CD7, Llama IgG2b Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

CD7 ELISA

Immobilized Anti-CD7 antibody, Mouse IgG1 at 1 μg/mL (100 μL/well) can bind FITC-Labeled Human CD7, Llama IgG2b Fc Tag (Cat. No. CD7-HF257) with a linear range of 0.039-0.625 ug/mL (QC tested).

Protocol

 

背景(Background)

T-cell antigen CD7 (CD7) is also known as GP40, LEU-9, TP41 and Tp40. CD7 is a protein that in humans is encoded by the CD7 gene, this gene encodes a transmembrane protein which is a member of the immunoglobulin superfamily. CD7 has been shown to interact with PIK3R1. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development.

 

前沿进展

 
 
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CD7靶点信息
英文全称:T-cell antigen CD7
中文全称:T细胞抗原CD7
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:23详情
最高研发阶段:临床二期
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