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Paguma larvata ACE2 / ACEH Protein, His Tag (SPR verified)

分子别名(Synonym)

ACE-2,ACEH,ACE2

表达区间及表达系统(Source)

Paguma larvata ACE2, His Tag (AC2-P5248) is expressed from human 293 cells (HEK293). It contains AA Gln 18 - Thr 740 (Accession # Q56NL1-1).

Predicted N-terminus: His

蛋白结构(Molecular Characterization)

Online(Gln 18 - Thr 740) Q56NL1-1

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 85.7 kDa. The protein migrates as 90-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Delivered as bulk protein in a 0.2 μm filtered solution of 50 mM Tris, 150 mM NaCl, pH7.5 with glycerol as protectant.

Contact us for customized product form or formulation.

运输(Shipping)

This product is supplied as sterile liquid solution and shipped frozen with dry ice.

存储(Storage)

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. The product MUST be stored at -70°C or lower upon receipt;
  2. -70°C for 3 months under sterile conditions.
 

电泳(SDS-PAGE)

Paguma larvata ACE2, His Tag (Cat. No. AC2-P5248) SDS-PAGE gel

Paguma larvata ACE2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Paguma larvata ACE2, His Tag (Cat. No. AC2-P5248) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 S1 protein, Fc Tag (Cat. No. S1N-C5255) with a linear range of 0.156-5 μg/mL (Routinely tested).

Protocol

Biotinylated Human  ELISA

Immobilized Paguma larvata ACE2, His Tag (Cat. No. AC2-P5248) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 S protein RBD, Fc Tag (Cat. No. SPD-C5255) with a linear range of 0.078-5 μg/mL (Routinely tested).

Protocol

活性(Bioactivity)-SPR

Biotinylated Human SPR

Paguma larvata ACE2, His Tag (Cat. No. AC2-P5248) immobilized on CM5 Chip can bind SARS S protein RBD, His Tag (Cat. No. SPD-S52H6) with an affinity constant of 0.101 μM as determined in a SPR assay (Biacore T200) (QC tested).

Protocol

Biotinylated Human SPR

Paguma larvata ACE2, His Tag (Cat. No. AC2-P5248) immobilized on CM5 Chip can bind SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H3) with an affinity constant of 29 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

Biotinylated Human SPR

Paguma larvata ACE2, His Tag (Cat. No. AC2-P5248) immobilized on CM5 Chip can bind SARS-CoV-2 S1 protein, His Tag (Cat. No. S1N-C52H4) with an affinity constant of 47.5 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

Biotinylated Human SPR

Paguma larvata ACE2, His Tag (Cat. No. AC2-P5248) immobilized on CM5 Chip can bind SARS S1 protein, His Tag (Cat. No. S1N-S52H5) with an affinity constant of 0.796 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

 

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded SARS-CoV-2 S protein RBD, Fc Tag (Cat. No. SPD-C5255) on Protein A Biosensor, can bind Paguma larvata ACE2, His Tag (Cat. No. AC2-P5248) with an affinity constant of 1.17 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

Angiotensin-converting enzyme 2 (ACE2) is also known as ACEH (ACE homolog), is an integral membrane protein with considerable homologous to ACE, which belongs to the peptidase M2 family. ACE2 is an exopeptidase that catalyses the conversion of angiotensin I to the nonapeptide angiotensin, or the conversion of angiotensin II to angiotensin 1-7. ACE2 may be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, ACE-2 serve as functional receptor for the spike glycoprotein of both coronaviruses. ACE2 is activated by chloride and fluoride, but not bromide and Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat. ACE2 is active from pH 6 to 9, and the optimum pH is 6.5 in the presence of 1 M NaCl.

 

前沿进展

货号/价格

AC2-P5248-50ug / ¥4200

AC2-P5248-1mg / ¥28000

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