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 >  Protein>IL-17F >ILF-C5243

Cynomolgus IL-17F Protein, His Tag

分子别名(Synonym)

IL-17F,Interleukin-17F,Cytokine ML-1

表达区间及表达系统(Source)

Cynomolgus IL-17F, His Tag (ILF-C5243) is expressed from human 293 cells (HEK293). It contains AA Arg 31 - Gln 163 (Accession # G7P4V0-1 ).

Predicted N-terminus: His

Request for sequence

蛋白结构(Molecular Characterization)

IL-17F Structure

This protein carries a polyhistidine tag at the N-terminus

The protein has a calculated MW of 16.8 kDa. The protein migrates as 22-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

IL-17F SDS-PAGE

Cynomolgus IL-17F, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

IL-17F ELISA

Immobilized Cynomolgus IL-17F, His Tag (Cat. No. ILF-C5243) at 2 μg/mL (100 μL/well) can bind Human IL-17 RA, Fc Tag (Cat. No. ILA-H5257) with a linear range of 0.005-0.313 μg/mL (QC tested).

Protocol

IL-17F ELISA

Immobilized Cynomolgus IL-17F, His Tag (Cat. No. ILF-C5243) at 2 μg/mL (100 μL/well) can bind Recombinant Human IL-17RC Isoform 1 Fc Chimera with a linear range of 0.5-16 ng/mL (Routinely tested).

Protocol

 

活性(Bioactivity)-BLI

IL-17F BLI

Loaded Human IL-17 RA, Fc Tag (Cat. No. ILA-H5257) on Protein A Biosensor, can bind Cynomolgus IL-17F, His Tag (Cat. No. ILF-C5243) with an affinity constant of 279 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

Interleukin-17F (IL17F) is also known as Interleukin-24 (IL24), Cytokine ML-1, is a secreted disulfide-linked homodimer which belongs to the IL-17 family. There are at least six members of the IL-17 family in humans and in mice: IL-17A, IL-17B, IL-17C, IL-17D, IL-17E (also called IL-25) and IL-17F (also called IL-24). IL-17 represents a family of structurally related cytokines that share a highly conserved C-terminal region but differ from one another in their N-terminal regions and in their distinct biological roles. IL-17F / IL-24 is expressed in activated, but not resting, CD4+ T-cells and activated monocytes. IL17F / Interleukin-24 stimulates the production of other cytokines such as IL-6, IL-8 and granulocyte colony-stimulating factor, and can regulate cartilage matrix turnover. IL17F / IL-24 stimulates PBMC and T-cell proliferation and Inhibits angiogenesis. Defects in IL17F are the cause of familial candidiasis type 6 (CANDF6).

 

前沿进展

 
 
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IL-17F靶点信息
英文全称:Interleukin-17F
中文全称:白介素-17F
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:4详情
最高研发阶段:临床三期
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