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SARS-CoV-2 (COVID-19) S protein (R683A, R685A), His Tag

分子别名(Synonym)

S protein,Spike glycoprotein,S glycoprotein,COVID-19

表达区间及表达系统(Source)

SARS-CoV-2 S protein (R683A, R685A), His Tag (SPN-C52H4) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213 (Accession # QHD43416.1 (R683A, R685A)).

Predicted N-terminus: Val 16

蛋白结构(Molecular Characterization)

Online(Val 16 - Pro 1213) QHD43416.1 (R683A, R685A)

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 134.6 kDa. The protein migrates as 170-220 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>85% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

SARS-CoV-2 S protein (R683A, R685A), His Tag (Cat. No. SPN-C52H4) SDS-PAGE gel

SARS-CoV-2 S protein (R683A, R685A), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 85%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized SARS-CoV-2 S protein (R683A, R685A), His Tag (Cat. No. SPN-C52H4) at 2 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 1-8 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized SARS-CoV-2 S protein (R683A, R685A), His Tag (Cat. No. SPN-C52H4) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-S protein RBD Antibody, Human IgG1 with a linear range of 0.2-8 ng/mL (Routinely tested).

Protocol

活性(Bioactivity)-SPR

Biotinylated Human SPR

Human ACE2, Fc Tag (Cat. No. AC2-H5257) Captured on CM5 chip via anti-human IgG Fc antibodies surface can bind SARS-CoV-2 S protein (R683A, R685A), His Tag (Cat. No. SPN-C52H4) with an affinity constant of 61.7 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Protocol

Biotinylated Human SPR

SARS-CoV-2 S protein (R683A, R685A), His Tag (Cat. No. SPN-C52H4) captured on CM5 Chip via anti-His antibody can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with an affinity constant of 2.91 nM as determined in SPR assay (Biacore 8K) (Routinely tested).

Protocol

 

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Human ACE2, Fc Tag (Cat. No. AC2-H5257) on Protein A Biosensor, can bind SARS-CoV-2 S protein (R683A, R685A), His Tag (Cat. No. SPN-C52H4) with an affinity constant of 22.5 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Biotinylated Human BLI

Loaded SARS-CoV-2 S protein (R683A, R685A), His Tag (Cat. No. SPN-C52H4) on AR2G Biosensor, can bind Human ACE2, His Tag (Cat. No. AC2-H52H8) with an affinity constant of 11.4 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

 

前沿进展

 
货号/价格

SPN-C52H4-100ug / ¥4800

SPN-C52H4-1mg (200ug X 5) / ¥35000

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