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Human 4-1BB Ligand / TNFSF9 (71-254) Protein, Fc Tag, active trimer (MALS verified)

分子别名(Synonym)

4-1BB Ligand,TNFSF9,CD137L

表达区间及表达系统(Source)

Human 4-1BB Ligand (71-254), Fc Tag, active trimer (41L-H5265) is expressed from human 293 cells (HEK293). It contains AA Arg 71 - Glu 254 (Accession # P41273-1) trimer Design.

Predicted N-terminus: Pro

蛋白结构(Molecular Characterization)

This protein carries a human IgG1 Fc tag at the N-terminus.

The protein has a calculated MW of 87.5 kDa. The protein migrates as 130-150 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human 4-1BB Ligand (71-254), Fc Tag, active trimer (Cat. No. 41L-H5265) SDS-PAGE gel

Human 4-1BB Ligand (71-254), Fc Tag, active trimer on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

Human 4-1BB Ligand (71-254), Fc Tag, active trimer (Cat. No. 41L-H5265) MALS images

The purity of Human 4-1BB Ligand (71-254), Fc Tag, active trimer (Cat. No. 41L-H5265) was more than 90% in HP-SEC, and around 185-215 kDa verified by SEC-MALS.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human 4‑1BB, His Tag (Cat. No. 41B-H5227) at 2 μg/mL (100 μL/well) can bind Human 4-1BB Ligand (71-254), Fc Tag, active trimer (Cat. No. 41L-H5265) with a linear range of 0.2-10 ng/mL (QC tested).

Protocol

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Human 4-1BB Ligand (71-254), Fc Tag, active trimer (Cat. No. 41L-H5265) on Protein A Biosensor, can bind Cynomolgus / Rhesus macaque 4-1BB, His Tag (Cat. No. 41B-C52H4) with an affinity constant of 10.3 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

活性(Bioactivity)-Cell based assay

Biotinylated Human CELL_BASE

Human 4-1BB Ligand (71-254), Fc Tag, active trimer (Cat. No. 41L-H5265) induce IL-8 secretion in HT1080 human CD137 cell line. The EC50 for this effect is 0.079 μg/mL (Routinely tested).

Protocol

 

背景(Background)

Tumor necrosis factor ligand superfamily member 9 (4-1BBL) is also known as 4-1BB ligand, CD137L or TNFSF9, which is a cytokine that binds to TNFRSF9. 4-1BBL is the high affinity ligand of 4-1BB. 4-1BBL induces the proliferation of activated peripheral blood T-cells. Also, 4-1BBL may have a role in activation-induced cell death (AICD). Furthermore, 4-1BBL may play a role in cognate interactions between T-cells and B-cells/macrophages. As for diseases, 4-1BBL is involved in cancers, infectious diseases and autoimmune diseases.

 

前沿进展

 
货号/价格

41L-H5265-100ug / ¥3500

41L-H5265-1mg (500ug X 2) / ¥23100

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