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Human Arginase 1 / ARG1 Protein, His Tag

分子别名(Synonym)

ARG1,Arginase-1

表达区间及表达系统(Source)

Human Arginase 1, His Tag (AR1-H5228) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Lys 322 (Accession # NP_000036).

Predicted N-terminus: Met 1

蛋白结构(Molecular Characterization)

Online(Met 1 - Lys 322) NP_000036

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 35.8 kDa. The protein migrates as 35-38 kDa under reducing (R) condition (SDS-PAGE).

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human Arginase 1, His Tag (Cat. No. AR1-H5228) SDS-PAGE gel

Human Arginase 1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

背景(Background)

Arginase-1 (ARG1) is also known as Liver-type arginase, Type I arginase, which belongs to the arginase family. Arginase-1 / ARG1 is a manganese-containing enzyme. The reaction catalyzed by this enzyme is: arginine + H2O → ornithine + urea. It is the final enzyme of the urea cycle. Defects in Arginase-1 / ARG1 are the cause of argininemia (ARGIN).

 

前沿进展

 
货号/价格

AR1-H5228-50ug / ¥3500

AR1-H5228-1mg (500ug X 2) / ¥19800

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