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 >  Protein>ErbB3 >ER3-H5257

Human ErbB3 / Her3 Protein, Mouse IgG2a Fc Tag (MALS verified)

分子别名(Synonym)

ERBB3,HER3,LCCS2,MDA-BF-1,MGC88033,c-erbB3,erbB3-S,p180-ErbB3,p45-sErbB3,p85-sErbB3

表达区间及表达系统(Source)

Human ErbB3, Mouse IgG2a Fc Tag (ER3-H5257) is expressed from human 293 cells (HEK293). It contains AA Ser 20 - Thr 643 (Accession # P21860-1 ).

Predicted N-terminus: Ser 20

Request for sequence

蛋白结构(Molecular Characterization)

ErbB3 Structure

This protein carries a mouse IgG2a Fc tag at the C-terminus

The protein has a calculated MW of 95.5 kDa. The protein migrates as 115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

ErbB3 SDS-PAGE

Human ErbB3, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

ErbB3 SEC-MALS

The purity of Human ErbB3, Mouse IgG2a Fc Tag (Cat. No. ER3-H5257) is more than 90% and the molecular weight of this protein is around 220-260 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

ErbB3 ELISA

Immobilized Human NRG1 Beta 1, Fc Tag, premium grade (Cat. No. NR1-H5268) at 2 μg/mL (100 μL/well) can bind Human ErbB3, Mouse IgG2a Fc Tag (Cat. No. ER3-H5257) with a linear range of 0.1-2 ng/mL (QC tested).

Protocol

 

背景(Background)

ErbB3,also known as Her3 (human epidermal growth factor receptor 3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain. It therefore can bind the ligand but cannot mediate the intracellular signal transduction through protein phosphorylation. However, it does form heterodimers with ErbB2 or other EGFR members responsible for tyrosine phosphorylation to give a receptor complex and initiate the related pathway, which lead to cell proliferation or differentiation. Overexpression of this protein has been reported in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.

 

前沿进展

 
 
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ErbB3靶点信息
英文全称:Receptor tyrosine-protein kinase erbB-3
中文全称:受体酪氨酸蛋白激酶erbB-3
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:21详情
最高研发阶段:批准上市
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