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 >  Protein>LILRB4 >LI4-H52H7

Human LILRB4 / CD85k / ILT3 Protein, His Tag (MALS verified)

分子别名(Synonym)

LILRB4,ILT3,LIR5,CD85K,HM18

表达区间及表达系统(Source)

Human LILRB4, His Tag (LI4-H52H7) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Glu 259 (Accession # AAH26309.1 ).

Predicted N-terminus: Gln 22

Request for sequence

蛋白结构(Molecular Characterization)

LILRB4 Structure

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 28.2 kDa. The protein migrates as 32-43 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

LILRB4 SDS-PAGE

Human LILRB4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

LILRB4 SEC-MALS

The purity of Human LILRB4, His Tag (Cat. No. LI4-H52H7) is more than 90% and the molecular weight of this protein is around 28-41 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

LILRB4 ELISA

Immobilized Human LILRB4, His Tag (Cat. No. LI4-H52H7) at 1 μg/mL (100 μL/well) can bind Anti-LILRB4 Antibody, Human IgG1 with a linear range of 0.1-2 ng/mL (QC tested).

Protocol

 

活性(Bioactivity)-SPR

LILRB4 SPR

Human ANGPTL7, His Tag (Cat. No. AN7-H52H5) immobilized on CM5 Chip can bind Human LILRB4, His Tag (Cat. No. LI4-H52H7) with an affinity constant of 4.67 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

 

背景(Background)

Leukocyte immunoglobulin-like receptor subfamily B member 4 (LILRB4) is also known as CD85 antigen-like family member K (CD85K), Immunoglobulin-like transcript 3 (ILT-3), Leukocyte immunoglobulin-like receptor 5 (LIR-5), Monocyte inhibitory receptor HM18, which belongs to the leukocyte immunoglobulin-like receptor (LIR) family. LILRB4 / CD85K contains 2 Ig-like C2-type (immunoglobulin-like) domains. CD85K is detected in monocytes, macrophages, dendritic cells, lung, natural killer cells and B-cells. LILRB4 / CD85K is receptor for class I MHC antigens. CD85K recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles, involved in the down-regulation of the immune response and the development of tolerance. LILRB4 interferes with TNFRSF5-signaling and NF-kappa-B up-regulation and inhibits receptor-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.

 

前沿进展

 
 
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LILRB4靶点信息
英文全称:Leukocyte immunoglobulin-like receptor subfamily B member 4
中文全称:免疫抑制受体LILRB4
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:5详情
最高研发阶段:临床二期
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