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 >  Protein>Fc gamma RIIA / CD32a >CDA-C82E5

Biotinylated Cynomolgus Fc gamma RIIA / CD32a Protein, His,Avitag™ (SPR & BLI & MALS verified)

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分子别名(Synonym)

CD32a,FCGR2A,CD32,FCG2 ,FCGR2A1,IGFR2

表达区间及表达系统(Source)

Biotinylated Cynomolgus CD32a, His,Avitag (CDA-C82E5) is expressed from human 293 cells (HEK293). It contains AA Gln 28 - Pro 208 (Accession # Q8SPW4-1 ).

Predicted N-terminus: Gln 28

Request for sequence

蛋白结构(Molecular Characterization)

Fc gamma RIIA / CD32a Structure

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)

The protein has a calculated MW of 23.9 kDa. The protein migrates as 30-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 12 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Fc gamma RIIA / CD32a SDS-PAGE

Biotinylated Cynomolgus CD32a, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Fc gamma RIIA / CD32a SEC-MALS

The purity of Biotinylated Cynomolgus CD32a, His,Avitag (Cat. No. CDA-C82E5) is more than 85% and the molecular weight of this protein is around 30-40 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-SPR

Fc gamma RIIA / CD32a SPR

Captured Biotinylated Cynomolgus CD32a, His,Avitag (Cat. No. CDA-C82E5) on Biotin CAP - Series S sensor Chip can bind MabThera® (Rituximab) with an affinity constant of 7.55 μM as determined in a SPR assay (Biacore T200) (QC tested).

Protocol

 

活性(Bioactivity)-BLI

Fc gamma RIIA / CD32a BLI

Loaded Biotinylated Cynomolgus CD32a, His,Avitag (Cat. No. CDA-C82E5) on SA Biosensor, can bind MabThera® (Rituximab) with an affinity constant of 2 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

Receptors for the Fc region of IgG (Fc γ R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc γ Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized.
There are three genes for human Fcγ RII /CD32 (A, B, and C) and one for mouse Fcγ RII B (CD32B). CD32 is a low affinity receptor for IgG. The activating isoform, CD32A, is expressed on monocytes, neutrophils, platelets and dendritic cells. CD32A is expressed on many immune cell types (macrophage, neutrophil, eosinophils, platelets, dendritic cells and Langerhan cells), where inhibitory ITIM­bearing receptors may also be coexpressed and co­engaged by specific ligands. CD32A delivers an activating signal upon ligand binding, and results in the initiation of inflammatory responses including cytolysis, phagocytosis, degranulation and cytokine production. The responses can be modulated by signals from the coexpressed inhibitory receptors such as CD32B, and the strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors.

 

前沿进展

 
 
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Fc gamma RIIA / CD32a靶点信息
英文全称:Low affinity immunoglobulin gamma Fc region receptor II-a
中文全称:低亲和力免疫球蛋白γFc区受体II-a
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:2详情
最高研发阶段:临床一期
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