|ID||Components||Size (96 tests)||Size (480 tests)|
|A006-214||Human LDL R||35 μg||160 μg|
|A007-214||Biotinylated Human PCSK9||10 μg||10 μg|
|A003-214||Streptavidin-HRP||10 μg||10 μg|
|PC9-NA003||Anti-PCSK9 Neutralizing Antibody||40 μg||200 μg|
Binding of Biotinylated Human PCSK9 to Immobilized Human LDL R in a Functional ELISA Assay.
Immobilized human LDL R protein at 3 μg/mL (100 μL/well) can bind biotinylated human PCSK9 with a linear range of 0.000625-0.02 μg/mL when detected by Streptavidin-HRP. Background was subtracted from data points before curve fitting.
Inhibition of PCSK9 [Biotinylated] : LDL R Binding by Anti-PCSK9 Neutralizing Antibody.
Serial dilutions of anti-PCSK9 neutralizing antibody (Catalog # PC9-NA003) (1:2 serial dilutions, from 20 μg/mL to 0.04 μg/mL) were added into PCSK9: biotinylated LDL R binding reactions. The assay was performed according to the above described protocol. Background was subtracted from data points prior to log transformation and curve fitting.