膜杰作
Star Staining
分子别名（Synonym）
SHPS1,SIRPA,CD172A,BIT,MFR,MYD1,P84,PTPNS1
表达区间及表达系统（Source）
PE-Labeled Human SIRP alpha, Fc Tag (Cat. No. SIA-HP252) is produced via site-specific conjugation of PE to Human SIRP alpha, Fc Tag under optimal conditions with a proprietary technology. Human SIRP alpha, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Arg 370 (Accession # P78324-1).
Predicted N-terminus: Glu 31
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 65.7 kDa.
应用说明（Application）
Flow Cytometry (Neutralizing assay), Please note that this product is NOT compatible to streptavidin detection system.
Conjugate
PE
Excitation Wavelength: 488 nm / 561 nm
Emission Wavelength: 575 nm
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
活性（Bioactivity）-FACS
Flow Cytometry assay shows that PE-Labeled Human SIRP alpha, Fc Tag (Cat. No. SIA-HP252) can bind to Jurkat cells overexpressing human CD47. 1 μL stock solution per million cells (QC tested).
Protocol
FACS analysis shows that the binding of PE-Labeled Human SIRP alpha, Fc Tag (Cat. No. SIA-HP252) to Jurkat cells overexpressing CD47 was inhibited by increasing concentration of neutralizing anti-human CD47 antibody. The IC50 is 0.37 μg/mL (QC tested).
Protocol
背景（Background）
Tyrosine-protein phosphatase non-receptor type substrate 1 (SHPS1) is also known as CD172 antigen-like family member A (CD172a), Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein alpha (SIRPA or SIRP alpha) or p84, is a member of the SIRP family, and also belongs to the immunoglobulin superfamily. SIRP alpha is Ubiquitous and highly expressed in brain. SIRPA / CD172a is immunoglobulin-like cell surface receptor for CD47 and acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA / SHPS-1 supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment and may play a key role in intracellular signaling during synaptogenesis and in synaptic function By similarity. SIRPA / MyD1 involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin and mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
