PE-Labeled Human SIRP alpha, Fc Tag (recommended for neutralizing assay) (Cat. No. SIA-HP252) is produced via conjugation of PE to Human SIRP alpha, Fc tag under optimal conditions. Human SIRP alpha, Fc tag is expressed in HEK293 cells containing AA Glu 31 - Arg 370 (Accession # P78324-1).
Predicted N-terminus: Glu 31
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 65.7 kDa.
Flow Cytometry (Neutralizing assay)
Excitation source: 488 nm spectral line, argon-ion laser
Excitation Wavelength: 488 nm
Emission Wavelength: 575 nm
Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
- 4-8°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Flow Cytometry assay shows that PE-Labeled Human SIRP alpha, Fc Tag (recommended for neutralizing assay) (Cat. No. SIA-HP252) can bind to 293T cells overexpressing human CD47. 1 μl stock solution per million cells (QC tested).
FACS analysis shows that the binding of PE-Labeled Human SIRP alpha, Fc Tag (recommended for neutralizing assay) (Cat. No. SIA-HP252) to 293T overexpressing CD47 was inhibited by increasing concentration of neutralizing anti-human CD47 antibody. The IC50 is 0.37 μg/ml (QC tested).
Tyrosine-protein phosphatase non-receptor type substrate 1 (SHPS1) is also known as CD172 antigen-like family member A (CD172a), Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein alpha (SIRPA or SIRP alpha) or p84, is a member of the SIRP family, and also belongs to the immunoglobulin superfamily. SIRP alpha is Ubiquitous and highly expressed in brain. SIRPA / CD172a is immunoglobulin-like cell surface receptor for CD47 and acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA / SHPS-1 supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment and may play a key role in intracellular signaling during synaptogenesis and in synaptic function By similarity. SIRPA / MyD1 involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin and mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.