|Catalog||Components||Size (96 tests)||Size (480 tests)|
|A004-214||Human CD47||35 μg||160 μg|
|A005-214||Biotinylated Human SIRP alpha||10 μg||10 μg|
|A003-214||Streptavidin-HRP||10 μg||10 μg|
|CD7-NA002||Anti-CD47 Neutralizing Antibody||20 μg||100 μg|
Binding of Biotinylated Human SIRP alpha to Immobilized Human CD47 in a Functional ELISA Assay.
Immobilized human CD47 protein at 3 μg/mL (100 μL/well) can bind biotinylated human SIRP alpha with a linear range of 0.004-0.125 μg/mL when detected by Streptavidin-HRP. Background was subtracted from data points before curve fitting.
Inhibition of CD47: SIRP alpha [Biotinylated] Binding by Anti-CD47 Neutralizing Antibody.
Serial dilutions of anti-CD47 neutralizing antibody (Catalog # CD7-NA002) (1:2 serial dilutions, from 10 μg/mL to 0.04 μg/mL) were added into CD47: biotinylated SIRP alpha binding reactions. The assay was performed according to the above described protocol. Background was subtracted from data points prior to log transformation and curve fitting.