登录 | 注册    关注公众号  
微信公众号
搜索
 >  Protein>Insulin R >INR-H52Ha

Human Insulin R / CD220 (28-944) Protein, His Tag (MALS & SPR verified)

分子别名(Synonym)

INSR,Insulin receptor,IR,CD220

表达区间及表达系统(Source)

Human Insulin R (28-944), His Tag (INR-H52Ha) is expressed from human 293 cells (HEK293). It contains AA His 28 - Lys 944 (Accession # P06213-2 ).

Predicted N-terminus: His 28

Request for sequence

蛋白结构(Molecular Characterization)

Insulin R Structure

This protein carries a polyhistidine tag at the C-terminus. The mature form of Insulin R a disulfide-linked heterotetramer composed of 2 alpha and 2 beta chains. Each α and β chain (partial fragment) has a calculated MW of 83.6 kDa and 22.8 kDa. The protein migrates as 120-130 kDa and 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Insulin R SDS-PAGE

Human Insulin R (28-944), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

Insulin R SEC-MALS

The purity of Human Insulin R (28-944), His Tag (Cat. No. INR-H52Ha) is more than 90% and the molecular weight of this protein is around 256-313 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Insulin R ELISA

Immobilized Human Insulin R (28-944), His Tag (Cat. No. INR-H52Ha) at 5 μg/mL (100 μL/well)can bind Human IGF-II, Fc Tag (Cat. No. IG2-H4260) with a linear range of 0.078-2.5 μg/mL (QC tested).

Protocol

Insulin R ELISA

Immobilized Human Insulin R (28-944), His Tag (Cat. No. INR-H52Ha) at 5 μg/mL (100 μL/well)can bind Insulin Biotin Conjugate with a linear range of 0.02-0.313 μg/mL (Routinely tested).

Protocol

 

活性(Bioactivity)-SPR

Insulin R SPR

Human Insulin R (28-944), His Tag (Cat. No. INR-H52Ha) captured on CM5 chip via anti-His antibody, can bind Human Insulin with an affinity constant of 249 nM as determined in a SPR assay (Biacore T200) (QC tested).

Protocol

 

背景(Background)

Insulin receptor (INSR) is also known as CD antigen CD220, which can be cleaved into the following 2 chains: Insulin receptor subunit alpha and Insulin receptor subunit beta. INSR is a tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains carry the insulin-binding regions, while the beta chains carry the kinase domain. Forms a hybrid receptor with IGF1R, the hybrid is a tetramer consisting of 1 alpha chain and 1 beta chain of INSR and 1 alpha chain and 1 beta chain of IGF1R. In addition to binding insulin, the insulin receptor can bind insulin-like growth factors (IGFI and IGFII). Isoform Short of INSR has a higher affinity for IGFII binding. When present in a hybrid receptor with IGF1R, INSR binds IGF1.

 

前沿进展

 
 
货号/价格
产品推荐
文档
联系电话:
+86 400-682-2521(全国)
010-53681107(北京)
021-50850665(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
Insulin R靶点信息
英文全称:Insulin receptor
中文全称:胰岛素受体
种类:Homo sapiens
上市药物数量:88详情
临床药物数量:104详情
最高研发阶段:批准上市
查看更多信息
前沿进展
点击查看详细

消息提示

请输入您的联系方式,再点击提交!

确定