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Human IL-12 R beta 1 / CD212 Protein, His Tag

分子别名(Synonym)

IL-12 R beta 1,IL12RB1,CD212,IL12R,IL12RB,IL-12RB1

表达区间及表达系统(Source)

Human IL-12 R beta 1, His Tag (ILB-H52H9) is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Glu 540 (Accession # P42701-1).

Predicted N-terminus: Cys 24

蛋白结构(Molecular Characterization)

Online(Cys 24 - Glu 540) P42701-1

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 59.0 kDa. The protein migrates as 66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human IL-12 R beta 1, His Tag (Cat. No. ILB-H52H9) SDS-PAGE gel

Human IL-12 R beta 1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human IL-12 R beta 1, His Tag (Cat. No. ILB-H52H9) at 2 μg/mL (100 μL/well) can bind Human IL-12B, Fc Tag (Cat. No. NK2-H5258) with a linear range of 0.078-1.25 μg/mL (QC tested).

Protocol

背景(Background)

The human IL-12 R subunit is a member of the cytokine receptor superfamily and the functional high-affinity IL-12R is composed of at least two beta-type cytokine receptor subunits, each independently exhibiting a low affinity for IL-12. IL-12 R beta 1 (Interleukin-12 receptor subunit beta-1) is also known as IL-12RB1, CD212. Functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade.

 

前沿进展

 
货号/价格

ILB-H52H9-100ug / ¥2600

ILB-H52H9-1mg (500ug X 2) / ¥16000

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