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Human GM-CSF R alpha Protein, Fc Tag

分子别名(Synonym)

GM-CSF R alpha,GM-CSF-R-alpha,GMCSFR-alpha,GMR-alpha,CSF2RA,CSF2R,CSF2RY,CDw116,CD116

表达区间及表达系统(Source)

Human GM-CSF R alpha, Fc Tag (GRA-H5255) is expressed from human 293 cells (HEK293). It contains AA Glu 23 - Gly 320 (Accession # P15509-1).

Predicted N-terminus: Glu 23

蛋白结构(Molecular Characterization)

Online(Glu 23 - Gly 320) P15509-1

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 60.9 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human GM-CSF R alpha, Fc Tag (Cat. No. GRA-H5255) SDS-PAGE gel

Human GM-CSF R alpha, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human GM-CSF, Tag Free (Cat. No. GMF-H4214) at 1 μg/mL (100 μL/well) can bind Human GM-CSF R alpha, Fc Tag (Cat. No. GRA-H5255) with a linear range of 0.5-16 ng/mL (QC tested).

Protocol

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Human GM-CSF R alpha, Fc Tag (Cat. No. GRA-H5255) on Protein A Biosensor, can bind ActiveMax® Human GM-CSF, Tag Free (Cat. No. GMF-H4214) with an affinity constant of 9.21 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

GM-CSF R alpha(Granulocyte-macrophage colony-stimulating factor receptor subunit alpha) is also known as CSF2RA, CD116, GMR-alpha. Low affinity receptor for granulocyte-macrophage colony-stimulating factor. Transduces a signal that results in the proliferation, differentiation, and functional activation of hematopoietic cells. Hereditary pulmonary alveolar proteinosis (hPAP) is a rare disorder of pulmonary surfactant accumulation and hypoxemic respiratory failure caused by mutations in CSF2RA (encoding the granulocyte/macrophage colony-stimulating factor [GM-CSF] receptor α-chain [CD116]), which results in reduced GM-CSF-dependent pulmonary surfactant clearance by alveolar macrophages.

 

前沿进展

 
货号/价格

GRA-H5255-100ug / ¥3000

GRA-H5255-1mg (500ug X 2) / ¥19800

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