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Human 2B4 / CD244 / SLAMF4 Protein, His Tag

分子别名(Synonym)

CD244,2B4,SLAMF4,NKR2B4,NAIL,h2B4

表达区间及表达系统(Source)

Human 2B4, His Tag (2B4-H5224) is expressed from human 293 cells (HEK293). It contains AA Cys 22 - Arg 221 (Accession # NP_057466).

Predicted N-terminus: Cys 22

蛋白结构(Molecular Characterization)

Online(Cys 22 - Arg 221) NP_057466

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 23.1 kDa. The protein migrates as 35-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human 2B4, His Tag (Cat. No. 2B4-H5224) SDS-PAGE gel

Human 2B4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human 2B4, His Tag (Cat. No. 2B4-H5224) at 5 μg/mL (100 μL/well) can bind Human CD48, Fc Tag (Cat. No. BC1-H5255) with a linear range of 0.078-1.25 μg/mL (QC tested).

Protocol

背景(Background)

Natural killer cell receptor 2B4 is also known as NK cell type I receptor protein 2B4 (NKR2B4 or h2B4), SLAM family member 4 (SLAMF4), Signaling lymphocytic activation molecule 4, CD antigen CD244. NKR2B4 / CD244 contains two Ig-like (immunoglobulin-like) domains. CD244 is expressed in spleen, PBL, followed by lung, liver, testis and small intestine. CD244 interacts with CD48. Following phosphorylation, CD244 is able to recruit PTPN11/SHP-2 and SH2D1A/SAP. SLAMF4 modulate other receptor-ligand interactions to enhance leukocyte activation. CD244/2B4 is the only heterophilic receptor of SLAM family.

 

前沿进展

 
货号/价格

2B4-H5224-100ug / ¥3150

2B4-H5224-1mg (500ug X 2) / ¥22500

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