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Human CD27 Ligand / CD70 Protein, His Tag

分子别名(Synonym)

CD70,CD27LG,TNFSF7,TNFSF7G,CD27L

表达区间及表达系统(Source)

Human CD27 Ligand, His Tag (CDL-H5246) is expressed from human 293 cells (HEK293). It contains AA Gln 39 - Pro 193 (Accession # P32970-1).

Predicted N-terminus: His

蛋白结构(Molecular Characterization)

Online(Gln 39 - Pro 193) P32970-1

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 19.0 kDa. The protein migrates as 30-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human CD27 Ligand, His Tag (Cat. No. CDL-H5246) SDS-PAGE gel

Human CD27 Ligand, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

>获取HPLC数据

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human CD27, Fc Tag (Cat. No. CD7-H5254) at 2 μg/mL (100 μL/well) can bind Human CD27 Ligand, His Tag (Cat. No. CDL-H5246) with a linear range of 0.013-0.1 μg/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Report

背景(Background)

Cluster of Differentiation 70 (CD70) is also known as CD27 ligand (CD27L / CD27LG), TNFSF7, TNFSF7G, is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and is a surface antigen found on activated T-and B-lymphocytes and mature dendritic cells. Binding of CD70 to its receptor CD27 induces in priming, effector functions, differentiation and memory formation of T-cells, and thus is invloved in the biological processes including T-cell activation, the proliferation of costimulated T-cells, as well as the generation of cytolytic T-cells. CD70 on T cells provides costimulatory signals that are required for T cell proliferation, clonal expansion and the promotion of effector T cell formation. CD70 on mouse B cell has been shown to inhibit terminal differentiation of activated B cells into plasma cells and enhances commitment to memory B cell responses. CD70 induces proliferation and IFNγ production, on NK cells.

 

前沿进展

 
货号/价格

CDL-H5246-100ug / ¥3000

CDL-H5246-1mg (500ug X 2) / ¥19800

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CD27 Ligand靶点信息
英文全称:CD70 antigen
中文全称:CD70抗原
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:9详情
最高研发阶段:临床二期
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