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Biotinylated Mouse Fc gamma RIIB / CD32b Protein, Avitag™,His Tag (SPR & BLI verified)

分子别名(Synonym)

FCGR2B,CD32b,CD32,FCG2,IGFR2,CDw32

表达区间及表达系统(Source)

Biotinylated Mouse CD32b, Avitag,His Tag (SPR & BLI verified) (CDB-M82E8) is expressed from human 293 cells (HEK293). It contains AA Thr 40 - Arg 217 (Accession # NP_001070657.1).

Predicted N-terminus: Thr 40

蛋白结构(Molecular Characterization)

Online(Thr 40 - Arg 217) NP_001070657.1

This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.

The protein has a calculated MW of 24.0 kDa. The protein migrates as 38-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Mouse CD32b, Avitag,His Tag (SPR & BLI verified) (Cat. No. CDB-M82E8) SDS-PAGE gel

Biotinylated Mouse CD32b, Avitag,His Tag (SPR & BLI verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

活性(Bioactivity)-SPR

Biotinylated Human SPR

Biotinylated Mouse CD32b, Avitag,His Tag (SPR & BLI verified) (Cat. No. CDB-M82E8) captured on Biotin CAP- Series S Sensor Chip can bind OKT3 with an affinity constant of 1.53 μM as determined in a SPR assay (Biacore T200) (QC tested).

Protocol

Biotinylated Human SPR

Report

 

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Biotinylated Mouse CD32b, Avitag,His Tag (SPR & BLI verified) (Cat. No. CDB-M82E8) on SA Biosensor, can bind MabThera® (Rituximab) with an affinity constant of 2.5 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

Receptors for the Fc region of IgG (Fc γ R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc γ Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized. There are three genes for human Fcγ RII /CD32 (A, B, and C) and one for mouse Fcγ RII B (CD32B). CD32 is a low affinity receptor for IgG. Low affinity immunoglobulin gamma Fc region receptor II-b (FCGR2B) is also known as CD32b, FCG2, IGFR2. CD32B is expressed on B cells and myeloid dendritic cells. Ligation of CD32B on B cells downregulates antibody production and may, in some circumstances, promote apoptosis. Co-ligation of CD32B on dendritic cells inhibits maturation and blocks cell activation. CD32B may also be a target for monoclonal antibody therapy for malignancies.

 

前沿进展

 
货号/价格

CDB-M82E8-25ug / ¥3500

CDB-M82E8-200ug / ¥13800

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