搜索
 >  其他蛋白>Glypican 3 >GP3-H52H4

Human Glypican 3 / GPC3 Protein, His Tag

分子别名(Synonym)

GPC3,OCI5,Glypican-3,GTR2-2,MXR7,DGSX,SDYS ,SGB,SGBS,SGBS1

表达区间及表达系统(Source)

Human Glypican 3, His Tag (GP3-H52H4) is expressed from human 293 cells (HEK293). It contains AA Gln 25 - His 559 (Accession # P51654-1).

Predicted N-terminus: Gln 25 & Ser 359

蛋白结构(Molecular Characterization)

Online(Gln 25 - His 559) P51654-1

This protein carries a polyhistidine tag at the C-terminus. This protein contains a furin-like convertase cleavage site, 355-RQYR-358, and will be partially processed into N and C-terminal fragment with calculated MW of 38.1 kDa and 24.6 kDa respectively. The protein migrates as 40 kDa, 32 kDa and 66-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human Glypican 3, His Tag (Cat. No. GP3-H52H4) SDS-PAGE gel

Human Glypican 3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human FGF basic, Tag Free (Cat. No. BFF-H4117) at 0.5 μg/mL (100 μL/well) can bind Human Glypican 3, His Tag (Cat. No. GP3-H52H4) with a linear range of 8-31 ng/mL (QC tested).

Protocol

Biotinylated Human SPR

Immobilized Human Glypican 3, His Tag (Cat. No. GP3-H52H4) at 0.5 μg/mL (100 μL/well) can bind Anti-Human GPC3 MAb with a linear range of 0.1-2 ng/mL (Routinely tested).

Protocol

活性(Bioactivity)-SPR

Biotinylated Human SPR

Anti-human GPC3 MAb (chimeric mouse-human IgG1) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human Glypican 3, His Tag (Cat. No. GP3-H52H4) with an affinity constant of 1.33 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

 

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Anti-Human GPC3 MAb (chimeric mouse-human IgG1) on AHC Biosensor, can bind Human Glypican 3, His Tag (Cat. No. GP3-H52H4) with an affinity constant of 1.48 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.

 

前沿进展

 
货号/价格

GP3-H52H4-100ug / ¥3200

GP3-H52H4-1mg (500ug X 2) / ¥18000

开发建议
文档
联系电话:
400-682-2521(全国)
010-57737274(北京)
021-26136428(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
techsupport@acrobiosystems.com

消息提示

请输入您的联系方式,再点击提交!

确定