PE-Labeled Human Mesothelin (296-580), His Tag (MSN-HP223) is produced via conjugation of PE to Human Mesothelin (296-580), His Tag under optimal conditions. Human Mesothelin (296-580), His Tag is expressed in HEK293 cells containing AA Glu 296 - Gly 580 (Accession # AAH09272).
Predicted N-terminus: Glu 296
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 33.0 kDa.
Excitation source: 488 nm spectral line, argon-ion laser
Excitation Wavelength: 488 nm
Emission Wavelength: 575 nm
Lyophilized from 0.22 μm filtered solution in 10 mM Tris, 150 mM NaCl, 0.1% BSA, pH8.2. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
CAR阳性表达率检测（Evaluation of CAR expression ）
FACS Analysis of Anti-MSLN CAR Expression
1e6 of the MSLN-CAR-293 cells were stained with 100 μl of 1:25 dilution (4 μL stock solution in 100 μl dilution buffer) of PE-Labeled Human Mesothelin (296-580), His Tag (Cat. No. MSN-HP223). PE Streptavidin was used as negative control (QC tested).
Mesothelin (MSLN) is also known as CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, which belongs to the mesothelin family. Mesothelin / MSLN can be proteolytically cleaved into the following two chains by a furin-like convertase: Megakaryocyte-potentiating factor (MPF) and the cleaved form of mesothelin. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro.