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PE-Labeled Human Mesothelin / MSLN (296-580) Protein, His Tag

分子别名(Synonym)

MSLN,Mesothelin,MPF

表达区间及表达系统(Source)

PE-Labeled Human Mesothelin (296-580), His Tag (Cat. No. MSN-HP223) is produced via conjugation of PE to Human Mesothelin (296-580), His Tag (Cat. No. MSN-H5223) under optimal conditions. Human Mesothelin (296-580), His Tag is expressed in HEK293 cells containing AA Glu 296 - Gly 580 (Accession # AAH09272).

Predicted N-terminus: Glu 296

蛋白结构(Molecular Characterization)

Online(Glu 296 - Gly 580) AAH09272

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 33.0 kDa.

Conjugate

PE

Excitation source: 488 nm spectral line, argon-ion laser

Excitation Wavelength: 488 nm

Emission Wavelength: 575 nm

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 10 mM Tris, 150 mM NaCl, 0.1% BSA, pH8.2. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.

CAR阳性表达率检测(Evaluation of CAR expression )

FACS Analysis of Anti-MSLN CAR Expression

CAR

1e6 of the MSLN-CAR-293 cells were stained with 100 μl of 1:25 dilution (4 μL stock solution in 100 μl dilution buffer) of PE-Labeled Human Mesothelin (296-580), His Tag (Cat. No. MSN-HP223). PE Streptavidin was used as negative control (QC tested).

Protocol

 

背景(Background)

Mesothelin (MSLN) is also known as CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, which belongs to the mesothelin family. Mesothelin / MSLN can be proteolytically cleaved into the following two chains by a furin-like convertase: Megakaryocyte-potentiating factor (MPF) and the cleaved form of mesothelin. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro.

 

前沿进展

 
货号/价格

MSN-HP223-50tests / ¥6000

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Mesothelin靶点信息
英文全称:Mesothelin
中文全称:间皮素
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:15详情
最高研发阶段:临床二期
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