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Biotinylated Human ICOS / CD278 (C136S, C137S) Protein, His,Avitag™ (recommended for biopanning)

分子别名(Synonym)

ICOS,CD278,AILIM,Inducible T-cell costimulator

表达区间及表达系统(Source)

Biotinylated Human ICOS (C136S, C137S), His,Avitag (recommended for biopanning) (ICS-H82E5) is expressed from human 293 cells (HEK293). It contains AA Glu 21 - Phe 141 (Accession # Q9Y6W8-1 (C136S, C137S)).

Predicted N-terminus: Glu 21

蛋白结构(Molecular Characterization)

Online(Glu 21 - Phe 141) Q9Y6W8-1 (C136S, C137S)

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

The protein has a calculated MW of 17.4 kDa. The protein migrates as 25-32 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human ICOS (C136S, C137S), His,Avitag (recommended for biopanning) (Cat. No. ICS-H82E5) SDS-PAGE gel

Biotinylated Human ICOS (C136S, C137S), His,Avitag (recommended for biopanning) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human B7-H2, Fc Tag (Cat. No. B72-H5254) at 5 μg/mL (100 μL/well) can bind Biotinylated Human ICOS (C136S, C137S), His,Avitag (recommended for biopanning) (Cat. No. ICS-H82E5) with a linear range of 2-78 ng/mL (QC tested).

Protocol

背景(Background)

Inducible T-cell costimulator (ICOS) is also known as Activation-inducible lymphocyte immunomediatory molecule (AILIM), CD278, which belongs to the CD28 family of immune costimulatory receptors consisting of CD28, CTLA-4 and PD-1. ICOS enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. CD278 / ICOS prevents the apoptosis of pre-activated T-cells and also plays a critical role in CD40-mediated class switching of immunoglobin isotypes.

 

前沿进展

 
货号/价格

ICS-H82E5-25ug / ¥3500

ICS-H82E5-200ug / ¥13800

开发建议
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联系电话:
400-682-2521(全国)
010-57737274(北京)
021-26136428(上海)
运输方式
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order.cn@acrobiosystems.com
技术支持邮箱:
techsupport@acrobiosystems.com
ICOS靶点信息
英文全称:Inducible T-cell costimulator
中文全称:诱导T细胞共刺激分子
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:7详情
最高研发阶段:临床三期
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