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Human CD39 / ENTPD1 Protein, His Tag (active enzyme)

分子别名(Synonym)

CD39,ENTPD1,NTPDase 1,Entpd1,Ecto-ATPDase 1,Ecto-ATPase 1

表达区间及表达系统(Source)

Human CD39, His Tag (CD9-H52H4) is expressed from human 293 cells (HEK293). It contains AA Thr 38 - Val 478 (Accession # P49961-1).

蛋白结构(Molecular Characterization)

Online(Thr 38 - Val 478) P49961-1

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 52.3 kDa. The protein migrates as 65-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris and NaCl, pH8.0. Normally Trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human CD39, His Tag (Cat. No. CD9-H52H4) SDS-PAGE gel

Human CD39, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)

Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-triphosphate (ATP). The specific activity is > 6,000 pmol/min/μg (QC tested).

Protocol

 

背景(Background)

CD39 is also known as Ectonucleoside triphosphate diphosphohydrolase 1, ENTPD1, NTPDase 1, Ecto-ATPDase 1, in the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation by hydrolyzing platelet-activating ADP to AMP. Hydrolyzes ATP and ADP equally well. NTPDase-1 was originally described as CD39, a B lymphocyte cell surface marker, but it is also present on the surface of natural killer cells, T cells, and some endothelial cells.Regulatory T cells(Tregs) mediate immunosuppression through multiple, non-redundant, cell-contact dependent and independent mechanisms, a growing body of evidence suggests an important role for the CD39-CD73-adenosine pathway. CD39 ectonucleotidase is the rate-limiting enzyme of a cascade leading to the generation of suppressive adenosine that alters CD4 and CD8 T cell and natural killer cell antitumor activities.

 

前沿进展

 
货号/价格

CD9-H52H4-100ug / ¥4000

CD9-H52H4-1mg (200ug X 5) / ¥26000

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CD39靶点信息
英文全称:Ectonucleoside triphosphate diphosphohydrolase 1
中文全称:外核苷三磷酸二磷酸水解酶-1
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:3详情
最高研发阶段:临床一期
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