分子别名（Synonym）
CD28,Tp44
表达区间及表达系统（Source）
Human / Cynomolgus / Rhesus macaque CD28, His Tag (CD8-H52Hc) is expressed from human 293 cells (HEK293). It contains AA Asn 19 - Pro 152 (Accession # P10747-1). In the region Asn 19 - Pro 152, the AA sequence of Human, Cynomolgus and Rhesus macaque CD28 are homologus.
Predicted N-terminus: Asn 19
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 17.0 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human / Cynomolgus / Rhesus macaque CD28, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Human / Cynomolgus / Rhesus macaque CD28, His Tag (Cat. No. CD8-H52Hc) was more than 90% and the molecular weight of this protein is around 55-65 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Monoclonal Anti-Human CD28 Antibody, Mouse IgG1 at 5 μg/mL (100 μL/well) can bind Human / Cynomolgus / Rhesus macaque CD28, His Tag (Cat. No. CD8-H52Hc) with a linear range of 0.1-1 ng/mL (QC tested).
Protocol
活性（Bioactivity）-BLI
Loaded Human B7-1, Fc Tag (HPLC-verified) (Cat. No. B71-H5259) on Protein A Biosensor, can bind Human / Cynomolgus / Rhesus macaque CD28, His Tag (Cat. No. CD8-H52Hc) with an affinity constant of 5.1 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human B7-2, Fc Tag (Cat. No. CD6-H5257) on Protein A Biosensor, can bind Human / Cynomolgus / Rhesus macaque CD28, His Tag (Cat. No. CD8-H52Hc) with an affinity constant of 4.5 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Monoclonal Anti-Human CD28 Antibody, Mouse IgG1 on AMC Biosensor, can bind Human / Cynomolgus / Rhesus macaque CD28, His Tag (Cat. No. CD8-H52Hc) with an affinity constant of 0.169 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
背景（Background）
T-cell-specific surface glycoprotein CD28 is also known as TP44, is a single-pass type I membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
