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Human / Cynomolgus / Rhesus macaque CD28 Protein, His Tag (MALS verified)

分子别名(Synonym)

CD28,Tp44

表达区间及表达系统(Source)

Human / Cynomolgus / Rhesus macaque CD28, His Tag (CD8-H52Hc) is expressed from human 293 cells (HEK293). It contains AA Asn 19 - Pro 152 (Accession # P10747-1). In the region Asn 19 - Pro 152, the AA sequence of Human, Cynomolgus and Rhesus macaque CD28 are homologus.

Predicted N-terminus: Asn 19

蛋白结构(Molecular Characterization)

Online(Asn 19 - Pro 152) P10747-1

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 17.0 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human / Cynomolgus / Rhesus macaque CD28, His Tag (Cat. No. CD8-H52Hc) SDS-PAGE gel

Human / Cynomolgus / Rhesus macaque CD28, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

Human / Cynomolgus / Rhesus macaque CD28, His Tag (Cat. No. CD8-H52Hc) MALS images

The purity of Human / Cynomolgus / Rhesus macaque CD28, His Tag (Cat. No. CD8-H52Hc) was more than 90% and the molecular weight of this protein is around 55-65 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Monoclonal Anti-Human CD28 Antibody, Mouse IgG1 at 5 μg/mL (100 μL/well) can bind Human / Cynomolgus / Rhesus macaque CD28, His Tag (Cat. No. CD8-H52Hc) with a linear range of 0.1-1 ng/mL (QC tested).

Protocol

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Human B7-1, Fc Tag (HPLC-verified) (Cat. No. B71-H5259) on Protein A Biosensor, can bind Human / Cynomolgus / Rhesus macaque CD28, His Tag (Cat. No. CD8-H52Hc) with an affinity constant of 5.1 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Biotinylated Human BLI

Loaded Human B7-2, Fc Tag (Cat. No. CD6-H5257) on Protein A Biosensor, can bind Human / Cynomolgus / Rhesus macaque CD28, His Tag (Cat. No. CD8-H52Hc) with an affinity constant of 4.5 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Biotinylated Human BLI

Loaded Monoclonal Anti-Human CD28 Antibody, Mouse IgG1 on AMC Biosensor, can bind Human / Cynomolgus / Rhesus macaque CD28, His Tag (Cat. No. CD8-H52Hc) with an affinity constant of 0.169 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

T-cell-specific surface glycoprotein CD28 is also known as TP44, is a single-pass type I membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.

 

前沿进展

 
货号/价格

CD8-H52Hc-100ug / ¥3500

CD8-H52Hc-1mg / ¥22000

开发建议
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运输方式
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CD28靶点信息
英文全称:T-cell-specific surface glycoprotein CD28
中文全称:T细胞特异性表面糖蛋白CD28
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:15详情
最高研发阶段:临床三期
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