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Human APRIL / TNFSF13 Protein, Fc Tag

分子别名(Synonym)

APRIL,TNFSF13,TALL-2,TRDL-1,CD256,TALL2,ZTNF2

表达区间及表达系统(Source)

Human APRIL, Fc Tag (APL-H5267) is expressed from human 293 cells (HEK293). It contains AA Ala 105 - Leu 250 (Accession # O75888-1).

Predicted N-terminus: Pro

蛋白结构(Molecular Characterization)

Online(Ala 105 - Leu 250) O75888-1

This protein carries a human IgG1 Fc tag at the N-terminus.

The protein has a calculated MW of 42.8 kDa. The protein migrates as 48 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human APRIL, Fc Tag (Cat. No. APL-H5267) SDS-PAGE gel

Human APRIL, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human BCMA, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. BCA-H5253) at 5 μg/mL (100 μL/well) can bind Human APRIL, Fc Tag (Cat. No. APL-H5267) with a linear range of 0.6-10 ng/mL (QC tested).

Protocol

Biotinylated Human SPR

Immobilized Human APRIL, Fc Tag (Cat. No. APL-H5267) at 5 μg/mL (100 μL/well) can bind Biotinylated Human TACI, Fc,Avitag (Cat. No. TAI-H82F6) with a linear range of 0.01-0.313 μg/mL (Routinely tested).

Protocol

背景(Background)

APRIL(a proliferation-inducing ligand) is also known as Tumor necrosis factor ligand superfamily member 13, TALL-2, TRDL-1, CD256, TNFFSF 13, cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. APRIL is a cytokine of the tumor necrosis factor family associated mainly with hematologic malignancies. The closely related TNF family ligands B cell activation factor (BAFF) and a proliferation-inducing ligand (APRIL) serve in the generation and maintenance of mature B-lymphocytes. Both BAFF and APRIL assemble as homotrimers that bind and activate several receptors that they partially share. BAFF-APRIL heteromers of different stoichiometries have distinct receptor-binding properties and activities. In addition, expression of APRIL was regulated by miR-145 in GC cells.

 

前沿进展

 
货号/价格

APL-H5267-100ug / ¥3500

APL-H5267-1mg (250ug X 4) / ¥23100

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APRIL靶点信息
英文全称:Tumor necrosis factor ligand superfamily member 13
中文全称:肿瘤坏死因子配体超家族成员13
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:7详情
最高研发阶段:BLA 申请
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