Integrin alpha 9 beta 1,ITGA9 & ITGB1
Human ITGA9&ITGB1 Heterodimer Protein, His Tag&Tag Free (IT1-H52W4) is expressed from human 293 cells (HEK293). It contains AA Tyr 30 - Trp 981 (ITGA9) & Gln 21 - Asp 728 (ITGB1) (Accession # Q13797-1 (ITGA9) & P05556-1 (ITGB1)).
Predicted N-terminus: Tyr 30 (ITGA9) & Gln 21 (ITGB1)
Human ITGA9&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA9 and ITGB1, has a calculated MW of 111.1 kDa (ITGA9) and 82.7 kDa (ITGB1). Subunit ITGA9 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus.
Less than 1.0 EU per μg by the LAL method.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Integrin alpha 9 beta 1 is one of twelve integrin family adhesion receptors that share the beta 1 (CD29) subunit. It is the non-covalent heterodimer of 150 kDa alpha 9 and 130 kDa beta 1 type I transmembrane glycoprotein subunits. The integrin alpha 9 beta 1 mediates cell adhesion to tenascin-C and VCAM-1 by binding to sequences distinct from the common integrin-recognition sequence, arginine-glycine-aspartic acid (RGD). A thrombin-cleaved NH(2)-terminal fragment of osteopontin containing the RGD sequence has recently been shown to also be a ligand for integrin alpha 9 beta 1.