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Human IGF-I R / CD221 Protein, His Tag

分子别名(Synonym)

IGF1R,IGFR,JTK13,CD221,MGC142170,MGC142172,MGC18216

表达区间及表达系统(Source)

Human IGF-I R, His Tag (IGR-H5229) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Asn 932 (Accession # P08069-1).

Predicted N-terminus: Glu 31 & Asp 741

蛋白结构(Molecular Characterization)

Online(Glu 31 - Asn 932) P08069-1

This protein carries a polyhistidine tag at the C-terminus. This protein contains a furin convertase cleavage site, 737-RKRR-740, and will be partially processed into N (α chain) and C-terminal fragment (partial β chain) with calculated MW of 81.0 kDa and 23.8 kDa respectively. The protein migrates as 36 kDa (partial β chain), 96-116 kDa (α chain) and 120-140 kDa (α chain & partial β chain) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human IGF-I R, His Tag (Cat. No. IGR-H5229) SDS-PAGE gel

Human IGF-I R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human IGF-I R, His Tag (Cat. No. IGR-H5229) at 5 μg/mL (100 μL/well) can bind Human IGF-I, Fc Tag (Cat. No. IG1-H4269) with a linear range of 0.04-0.313 μg/mL (QC tested).

Protocol

Biotinylated Human SPR

Immobilized Human IGF-I R, His Tag (Cat. No. IGR-H5229) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IGF-II, Avitag,Fc Tag (Cat. No. IG2-H82F9) with a linear range of 0.156-5 μg/mL (Routinely tested).

Protocol

背景(Background)

The Insulin-like Growth Factor 1 Receptor (IGF1) is also known as CD221, JTK13. and is a transmembrane receptor that is activated by IGF-1 and by the related growth factor IGF-2. It belongs to the large class of tyrosine kinase receptors. This receptor mediates the effects of IGF-1, which is a polypeptide protein hormone similar in molecular structure to insulin. IGF1R is make up of two alpha subunits and two beta subunits ,the Both the α and β subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds to form a functional transmembrane αβ chain.The α chains are located extracellularly while the β subunit spans the membrane and are responsible for intracellular signal transduction upon ligand stimulation. IGF1R have a binding site for ATP, which is used to provide the phosphates for autophosphorylation. There is a 60% homology between IGF1R and the insulin receptor. In response to ligand binding, the α chains induce the tyrosine autophosphorylation of the β chains. This event triggers a cascade of intracellular signaling that, while somewhat cell type specific, often promotes cell survival and cell proliferation.

 

前沿进展

 
货号/价格

IGR-H5229-100ug / ¥3500

IGR-H5229-1mg / ¥23100

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IGF-I R靶点信息
英文全称:Insulin-like growth factor I receptor
中文全称:胰岛素样生长因子-I受体
种类:Homo sapiens
上市药物数量:7详情
临床药物数量:32详情
最高研发阶段:批准上市
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