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Biotinylated Human GITR / TNFRSF18 Protein, Fc,Avitag™

分子别名(Synonym)

AITR,GITR,TNFRSF18,CD357

表达区间及表达系统(Source)

Biotinylated Human GITR, Fc,Avitag (GIR-H82F7) is expressed from human 293 cells (HEK293). It contains AA Gln 26 - Glu 161 (Accession # Q9Y5U5-1).

Predicted N-terminus: Gln 26

蛋白结构(Molecular Characterization)

Online(Gln 26 - Glu 161) Q9Y5U5-1

This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).

The protein has a calculated MW of 43.3 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human GITR, Fc,Avitag (Cat. No. GIR-H82F7) SDS-PAGE gel

Biotinylated Human GITR, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human GITR Ligand, His Tag (Cat. No. GIL-H5249) at 2 μg/mL (100 μL/well) can bind Biotinylated Human GITR, Fc,Avitag (Cat. No. GIR-H82F7) with a linear range of 0.4-6 ng/mL (QC tested).

Protocol

背景(Background)

Glucocorticoid-induced TNFR-related protein (GITR) is also known as Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), activation-inducible TNFR family receptor (AITR), CD antigen CD357, which is a member of the tumor necrosis factor receptor (TNF-R) superfamily. GITR is receptor for TNFSF18, which seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. GITR also mediated NF-kappa-B activation via the TRAF2/NIK pathway.

 

前沿进展

 
货号/价格

GIR-H82F7-25ug / ¥3500

GIR-H82F7-200ug / ¥13800

开发建议
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400-682-2521(全国)
010-57737274(北京)
021-26136428(上海)
运输方式
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order.cn@acrobiosystems.com
技术支持邮箱:
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GITR靶点信息
英文全称:Glucocorticoid-induced tumor necrosis factor receptor-related protein
中文全称:糖皮质激素诱导的肿瘤坏死因子受体相关蛋白
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:12详情
最高研发阶段:临床二期
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