Rhesus macaque CD155, His Tag (CD5-R52H5) is expressed from human 293 cells (HEK293). It contains AA Asp 28 - Asn 343 (Accession # Q0MSE6-1).
Predicted N-terminus: Asp 28
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 36.3 kDa. The protein migrates as 48-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Rhesus macaque CD155, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Rhesus macaque CD155, His Tag (Cat. No. CD5-R52H5) at 5 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus macaque TIGIT, Fc Tag (Cat. No. TIT-C5254) with a linear range of 0.02-0.313 μg/mL (QC tested).
Immobilized Rhesus macaque CD155, His Tag (Cat. No. CD5-R52H5) at 5 μg/mL (100 μL/well) can bind Biotinylated Human DNAM-1, Fc,Avitag (Cat. No. DN1-H82F9) with a linear range of 10-156 ng/mL (Routinely tested).
Loaded Human DNAM-1, Fc Tag (Cat. No. DN1-H5257) on Protein A Biosensor, can bind Rhesus macaque CD155, His Tag (Cat. No. CD5-R52H5) with an affinity constant of 0.47 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
CD155 (cluster of differentiation 155) also known as the poliovirus receptor is a protein that is encoded by the PVR gene. CD155 is a Type I transmembrane glycoprotein in the immunoglobulin superfamily. Commonly known as Poliovirus Receptor (PVR) due to its involvement in the cellular poliovirus infection in primates, CD155's normal cellular function is in the establishment of intercellular adherens junctions between epithelial cells. The role of CD155 in the immune system is unclear, though it may be involved in intestinal humoral immune responses. Subsequent data has also suggested that CD155 may also be used to positively select MHC-independent T cells in the thymus.