NECTIN4,LNIR, PRR4, PVRL4
Human Nectin-4, His Tag (NE4-H52H3) is expressed from human 293 cells (HEK293). It contains AA Gly 32 - Ser 349 (Accession # Q96NY8-1).
Predicted N-terminus: Gly 32
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 36.0 kDa. The protein migrates as 40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human Nectin-4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human Nectin-4, His Tag (Cat. No. NE4-H52H3) at 5 μg/mL (100 μL/well) can bind Human PVRL1, Fc Tag with a linear range of 0.01-0.313 μg/mL (QC tested).
Immobilized Human Nectin-4, His Tag (Cat. No. NE4-H52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal anti-Nectin-4 antibody, Human IgG1 with a linear range of 0.006-0.6 μg/mL (Routinely tested).
Loaded Human Nectin-1, Fc Tag (Cat. No. PV1-H5253) on Protein A Biosensor, can bind Human Nectin-4, His Tag (Cat. No. NE4-H52H3) with an affinity constant of 0.271 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Human Nectin-4, His Tag (Cat. No. NE4-H52H3) on HIS1K Biosensor, can bind Human Nectin-1, Fc Tag (Cat. No. PV1-H5253) with an affinity constant of 0.24 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Nectin-4 (gene name PVRL4, poliovirus receptor-like 4) is a 66 kDa type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. Nectins are cell adhesion molecules that play a key role in various biological processes such as polarity, proliferation, differentiation and migration, for epithelial, endothelial, immune and neuronal cells, during development and adult life. Nectin-4 is a tumor-associated antigen in 50%, 49% and 86%o of breast, ovarian and lung carcinomas, respectively, mostly on tumors of bad prognosis. Its expression is not detected in the corresponding normal tissues.