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 >  Protein>IL-3 R alpha >ILA-H5255

Human IL-3 R alpha / CD123 Protein, Llama IgG2b Fc Tag, low endotoxin

分子别名(Synonym)

IL3R,IL3RA,IL-3Ra,IL-3R-alpha,IL3RAY,IL3RX,IL3RY,CD123 antigen,CD123,hIL3Ra,hIL-3Ra,MGC34174,IL-3 R alpha

表达区间及表达系统(Source)

Human IL-3 R alpha Protein, Llama IgG2b Fc Tag (ILA-H5255) is expressed from human 293 cells (HEK293). It contains AA Thr 19 - Arg 305  (Accession # P26951-1).

Predicted N-terminus: Thr 19

Request for sequence

蛋白结构(Molecular Characterization)

IL-3 R alpha Structure

This protein carries a llama IgG2b Fc tag at the C-terminus

The protein has a calculated MW of 60.9 kDa. The protein migrates as 45 kDa, 55 kDa and 80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.01 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

IL-3 R alpha SDS-PAGE

Human IL-3 R alpha Protein, Llama IgG2b Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-BLI

IL-3 R alpha BLI

Loaded ActiveMax® Human IL-3, His Tag (Cat. No. IL3-H52H9) on NTA Biosensor, can bind Human IL-3 R alpha Protein, Llama IgG2b Fc Tag (Cat. No.ILA-H5255) with an affinity constant of 16.9 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

Protocol

 

背景(Background)

Interleukin 3 receptor alpha (low affinity) (IL3RA), also known as CD123 (Cluster of Differentiation 123) is a 70-kD glycoprotein member of the hematopoietin receptor superfamily. This protein associates with a beta subunit common to the receptors for IL-5 and granulocyte-macrophage colony-stimulating factor (GM-CSF) to form a high-affinity receptor for IL-3. The interleukin-3 receptor α chain (CD123) has been identified as a potential immunotherapeutic target because it is overexpressed in AML compared with normal hematopoietic stem cells.

 

前沿进展

 
 
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IL-3 R alpha靶点信息
英文全称:Interleukin-3 receptor subunit alpha
中文全称:白细胞介素-3受体α亚基
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:33详情
最高研发阶段:批准上市
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