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Biotinylated Human CTGF / CCN2 Protein, His,Avitag™ (recommended for biopanning)

分子别名(Synonym)

CCN2,NOV2,HCS24,IGFBP8,IBP-8,IGFBP-8,IGF-binding protein 8

表达区间及表达系统(Source)

Biotinylated Human CTGF, His,Avitag (recommended for biopanning) (CTF-H82E6) is expressed from human 293 cells (HEK293). It contains AA Gln 27 - Ala 349 (Accession # Q5M8T4-1).

Predicted N-terminus: Gln 27

蛋白结构(Molecular Characterization)

Online(Gln 27 - Ala 349) Q5M8T4-1

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

The protein has a calculated MW of 39.2 kDa. The protein migrates as 40-42 kDa and 43-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Potassium glutamate and Arginine, pH7.0. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human CTGF, His,Avitag (recommended for biopanning) (Cat. No. CTF-H82E6) SDS-PAGE gel

Biotinylated Human CTGF, His,Avitag (recommended for biopanning) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Biotinylated Human CTGF, His,Avitag (recommended for biopanning) (Cat. No. CTF-H82E6) at 2 μg/mL (100 μL/well) on streptavidin precoated (0.2 μg/well) plate, can bind Monoclonal Anti-Human CTGF Antibody, Human IgG1 with a linear range of 0.6-5 ng/mL (QC tested).

Protocol

背景(Background)

Connective Tissue Growth Factor (CTGF), also known as CCN2, is a member of the CCN (CCN1-6) family of modular matricellular proteins. Like other CCN proteins, mature human CTGF consists of IGF-binding protein domain, a vWF-C domain, a TSP-1 domain, and a cysteine knot heparin-binding domain. CTGF promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis. Analysis of CCN2 function in vivo has focused primarily on its key role as a mediator of excess ECM synthesis in multiple fibrotic diseases.

 

前沿进展

 
货号/价格

CTF-H82E6-25ug / ¥3500

CTF-H82E6-200ug (100ug X 2) / ¥13800

开发建议
文档
联系电话:
400-682-2521(全国)
010-57737274(北京)
021-26136428(上海)
运输方式
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order.cn@acrobiosystems.com
技术支持邮箱:
techsupport@acrobiosystems.com
CTGF靶点信息
英文全称:Connective Tissue Growth Factor
中文全称:结缔组织生长因子
种类:Homo sapiens
上市药物数量:2详情
临床药物数量:9详情
最高研发阶段:批准上市
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