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 >  Protein>CTLA-4 >CT4-C5227

Cynomolgus / Rhesus macaque CTLA-4 Protein, His Tag (MALS verified)

分子别名(Synonym)

CTLA4,CD152

表达区间及表达系统(Source)

Cynomolgus / Rhesus macaque CTLA-4, His Tag (CT4-C5227) is expressed from human 293 cells (HEK293). It contains AA Ala 37 - Ser 160 (Accession # G7PL88-1). In the region Ala 37 - Ser 160, the AA sequence of Cynomolgus and Rhesus macaque CTLA-4 are homologus.

Predicted N-terminus: Ala 37

Request for sequence

蛋白结构(Molecular Characterization)

Online(Ala 37 - Ser 160) G7PL88-1

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 14.1 kDa. The protein migrates as 19-24 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Cynomolgus / Rhesus macaque CTLA-4, His Tag (Cat. No. CT4-C5227) SDS-PAGE gel

Cynomolgus / Rhesus macaque CTLA-4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Cynomolgus / Rhesus macaque CTLA-4, His Tag (Cat. No. CT4-C5227) MALS images

The purity of Cynomolgus / Rhesus macaque CTLA-4, His Tag (Cat. No. CT4-C5227) is more than 95% and the molecular weight of this protein is around 22-32 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Cynomolgus / Rhesus macaque B7-1, Fc Tag (Cat. No. CD0-C5252) at 2 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus macaque CTLA-4, His Tag (Cat. No. CT4-C5227) with a linear range of 0.06-0.5ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Cynomolgus / Rhesus macaque B7-2, Fc Tag (Cat. No. CD6-C5254) at 2 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus macaque CTLA-4, His Tag (Cat. No. CT4-C5227) with a linear range of 4-31 ng/mL (Routinely tested).

Protocol

背景(Background)

CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis.

 

 

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CTLA-4靶点信息
英文全称:Cytotoxic T-Lymphocyte-Associated Antigen 4
中文全称:细胞毒性T淋巴细胞相关抗原4
种类:Homo sapiens
上市药物数量:3详情
临床药物数量:57详情
最高研发阶段:批准上市
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