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 >  Protein>IGF-I R >IGR-R5224

Rat IGF-I R / CD221 Protein, His Tag (MALS verified)

分子别名(Synonym)

IGF1R,IGFR,JTK13,CD221,MGC142170,MGC142172,MGC18216

表达区间及表达系统(Source)

Rat IGF-I R, His Tag (IGR-R5224) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - His 936 (Accession # P24062-1 ).

Predicted N-terminus: Glu 31

Request for sequence

蛋白结构(Molecular Characterization)

IGF-I R Structure

This protein carries a polyhistidine tag at the C-terminus.

This protein contains a furin convertase cleavage site, 738-RRRR-741, and will be partially processed into N (α chain) and C-terminal fragment (partial β chain) with calculated MW of 81.3 kDa and 24.2 kDa respectively. The protein migrates as 45-50 kDa (partial β chain), 96-115 kDa (α chain) and 120 kDa (α chain & partial β chain) under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

IGF-I R SDS-PAGE

Rat IGF-I R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

IGF-I R SEC-MALS

The purity of Rat IGF-I R, His Tag (Cat. No. IGR-R5224) is more than 85% and the molecular weight of this protein is around 254-310 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

IGF-I R ELISA

Immobilized Rat IGF-I R, His Tag (Cat. No. IGR-R5224) at 5 μg/mL (100 μL/well) can bind Human IGF-I, Fc Tag with a linear range of 5-39 ng/mL (QC tested).

Protocol

 

背景(Background)

The Insulin-like Growth Factor 1 Receptor (IGF1) is also known as CD221, JTK13. and is a transmembrane receptor that is activated by IGF-1 and by the related growth factor IGF-2. It belongs to the large class of tyrosine kinase receptors. This receptor mediates the effects of IGF-1, which is a polypeptide protein hormone similar in molecular structure to insulin. IGF1R is make up of two alpha subunits and two beta subunits ,the Both the α and β subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds to form a functional transmembrane αβ chain.The α chains are located extracellularly while the β subunit spans the membrane and are responsible for intracellular signal transduction upon ligand stimulation. IGF1R have a binding site for ATP, which is used to provide the phosphates for autophosphorylation. There is a 60% homology between IGF1R and the insulin receptor. In response to ligand binding, the α chains induce the tyrosine autophosphorylation of the β chains. This event triggers a cascade of intracellular signaling that, while somewhat cell type specific, often promotes cell survival and cell proliferation.

 

前沿进展

 
 
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IGF-I R靶点信息
英文全称:Insulin-like growth factor I receptor
中文全称:胰岛素样生长因子-I受体
种类:Homo sapiens
上市药物数量:6详情
临床药物数量:23详情
最高研发阶段:批准上市
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