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Human Serum Albumin Protein, His Tag, low endotoxin

分子别名(Synonym)

Serum albumin,ALB,Alb

表达区间及表达系统(Source)

Human Serum Albumin, His Tag, low endotoxin (HSA-H522a) is expressed from human 293 cells (HEK293). It contains AA Asp 25 - Leu 609 (Accession # P02768-1).

Predicted N-terminus: Asp 25

蛋白结构(Molecular Characterization)

Online(Asp 25 - Leu 609) P02768-1

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 67.4 kDa. The protein migrates as 67-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.01 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human Serum Albumin, His Tag, low endotoxin (Cat. No. HSA-H522a) SDS-PAGE gel

Human Serum Albumin, His Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Biotinylated Human FCGRT&B2M Heterodimer Protein, His,Avitag (Cat. No. FCM-H82W7) on SA Biosensor, can bind Human Serum Albumin, His Tag, low endotoxin (Cat. No. HSA-H522a) with an affinity constant of 0.596 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

serum albumin (SA) is also known as ALB, which is the main protein of plasma and has a good binding capacity for water,Ca2+,Na+,K+,fatty acids,hormones, bilirubin and drugs.The main function of SA is the regulation of the colloidal osmotic pressure of blood. As Major zinc transporter in plasma, SA typically binds about 80% of all plasma zinc. A variant structure of albumin could lead to increased binding of zinc resulting in an asymptomatic augmentation of zinc concentration in the blood. Defects in serum albumin can cause familial dysalbuminemic hyperthyroxinemia which is a form of euthyroid hyperthyroxinemia that is due to increased affinity of serum albumin for T4. It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.

 

前沿进展

 
货号/价格

HSA-H522a-1mg (200ug X 5) / ¥4000

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