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ELISA Assay Kit for Anti-CTLA-4 h-mAb in Mouse Serum

Cat. No. Components Size (96 tests) Size (480 tests)
PK801-213 Human CTLA-4 15 μg 60 μg
PK802-213 Biotinylated anti-CTLA-4 antibody 10 μg 10 μg
PK803-213 Streptavidin-HRP 5 μg 10 μg
PK804-213 Anti-CTLA-4 antibody 30 μg 30 μg

Background/背景

How a therapeutic antibody is metabolized in the body is pertinently relevant to its efficacy. Therefore, pharmacokinetics study is an important part of the drug development.

CTLA-4 is probably the most targeted molecule in today’s pharmaceutical industry, thanking to the clinical success of Yervoy®. Many investigational anti-CTLA-4 mAbs are being developed. There is a growing need for a standard assay that can be used to facilitate the study of their pharmacokinetics.

Assay Principles/原理

The enclosed ELISA assay kit for anti-CTLA-4 h-mAb in mouse serum is based on an enzyme immunoassay (ELISA) between recombinant CTLA-4 protein (ECD) and a biotinylated anti-CTLA-4 monoclonal antibody. The method employs the principle of competitive ELISA, allowing quantification of monoclonal anti-CTLA-4 antibodies in mouse serum.

The assay only involves the following steps:

1. Coat the plate with human CTLA-4;

2. Add the mixture of your sample and biotinylated anti-CTLA-4 antibody;

3. Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate;

4. Record the OD readings and analyze the serum concentration.

Reconstitution/重构方法

See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Advantages/产品优势

Wide Coverage

There is no requirement for the species and subtype of the antibody.

Easy Process

There is no longer a need to generate anti-drug antibodies for PK studies.

Clean Result

The use of the biotinylated antibody in the competitive ELISA significantly alleviates the issue of background noise issue that often occurs in traditional ELISA method.

Storage/存储

Upon receipt, please store all items at -20℃.

After reconstitution, the stock solution should be kept at -80℃.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. Room temperature (RT) for 1 month in lyophilized state;
  2. -20℃ for 6 months in lyophilized state;
  3. -80℃ for 4 months under sterile conditions after reconstitution.

Typical Data/典型数据

前沿进展

货号/价格

ECM-V1-96tests / ¥3160

ECM-V1-480tests / ¥11360

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CTLA-4靶点信息
英文全称:Cytotoxic T-Lymphocyte-Associated Antigen 4
中文全称:细胞毒性T淋巴细胞相关抗原4
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:14详情
最高研发阶段:批准上市
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