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Human CD30 Ligand / TNFSF8 Protein, Mouse IgG2a Fc Tag, low endotoxin

分子别名(Synonym)

CD30-L,CD153,TNFSF8,CD30L,CD30LG,CD153 antigen,CD30 antigen ligand,CD30 Ligand

表达区间及表达系统(Source)

Human CD30 Ligand, Mouse IgG2a Fc Tag, low endotoxin (CDL-H525b) is expressed from human 293 cells (HEK293). It contains AA Gln 63 - Asp 234 (Accession # P32971-1).

Predicted N-terminus: Glu

蛋白结构(Molecular Characterization)

Online(Gln 63 - Asp 234) P32971-1

This protein carries a mouse IgG2a Fc tag at the N-terminus.

The protein has a calculated MW of 46.5 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human CD30 Ligand, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CDL-H525b) SDS-PAGE gel

Human CD30 Ligand, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human CD30, His Tag (Cat. No. CD0-H5229) at 2 μg/mL (100 μL/well) can bind Human CD30 Ligand, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CDL-H525b) with a linear range of 1-25 ng/mL (QC tested).

Protocol

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Biotinylated Human CD30, Avitag,His Tag (Cat. No. CD0-H82E6) on SA Biosensor, can bind Human CD30 Ligand, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CDL-H525b) with an affinity constant of 114 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

活性(Bioactivity)-FACS

Biotinylated Human FACS

FACS analysis shows that Human CD30 Ligand, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CDL-H525b) can bind to K562-CD30 cells surface CD30. The concentration of Human CD30 Ligand is 0.1 μg/mL (Routinely tested).

Protocol

Biotinylated Human FACS

FACS analysis shows that the binding of Human CD30 Ligand, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CDL-H525b) to K562-CD30 cells surface CD30 was inhibited by increasing concentration of neutralizing anti-CD30 antibody. The concentration of Human CD30 Ligand used is 0.1 μg/mL. The IC50 is 0.2064 μg/mL (Routinely tested).

Protocol

 

背景(Background)

CD30 ligand (CD30L) and CD30 are interacting cellsurface glycoproteins that are members of the tumour necrosis factor (TNF) and tumour necrosis factor receptor (TNFR) superfamilies. In humans, CD30L is expressed at high levels on activated T cells. There is conflicting evidence with regard to cell surface expression of CD30L on primary human B cells. An initial report of constitutive CD30L expression on peripheral blood B cells33 has not been substantiated.

 

前沿进展

 
货号/价格

CDL-H525b-100ug / ¥3500

CDL-H525b-1mg (500ug X 2) / ¥23100

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