登录 | 注册    关注公众号  
微信公众号
搜索
 >  Protein>CD48 >BC1-H5258

Human CD48 / SLAMF2 Protein, Mouse IgG2a Fc Tag, low endotoxin

分子别名(Synonym)

CD48,BCM1,SLAMF2,BLAST,BLAST1,MEM-102,TCT.1,BCM-1,SLAMF-2,BLAST-1

表达区间及表达系统(Source)

Human CD48 Protein, Mouse IgG2a Fc Tag (BC1-H5258) is expressed from human 293 cells (HEK293). It contains AA Gln 27 - Ser 220 (Accession # P09326-1 ).

Predicted N-terminus: Gln 27

Request for sequence

蛋白结构(Molecular Characterization)

CD48 Structure

This protein carries a mouse IgG2a Fc tag at the C-terminus

The protein has a calculated MW of 49.2 kDa. The protein migrates as 64-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

CD48 SDS-PAGE

Human CD48 Protein, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

CD48 ELISA

Immobilized Human 2B4, Fc Tag (Cat. No. 2B4-H5255) at 2 μg/mL (100 μL/well) can bind Human CD48 Protein, Mouse IgG2a Fc Tag (Cat. No. BC1-H5258) with a linear range of 2-31 ng/mL (QC tested).

Protocol

CD48 ELISA

Immobilized Biotinylated Human 2B4 Protein, Avitag,His Tag (Cat. No. 2B4-H82E9) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.2 μg/well) plate, can bind Human CD48 Protein, Mouse IgG2a Fc Tag (Cat. No. BC1-H5258) with a linear range of 0.3-5 ng/mL (Routinely tested).

Protocol

 

背景(Background)

CD48 antigen is also known as Signaling lymphocytic activation molecule 2, SLAM family member 2 (SLAMF2), Leukocyte antigen MEM-102, BCM1 surface antigen, B-lymphocyte activation marker BLAST-1. CD48 is a 65 kDa GPI-linked protein which belongs to the CD2 subfamily of immunoglobulin superfamily molecules. CD48 antigen contains two Ig-like C2-type (immunoglobulin-like) domains and two Ig-like V-type (immunoglobulin-like) domain. CD48 interacts with CD244 in an heterophilic manner. CD48 is ligand for CD2. CD48 might facilitate interaction between activated lymphocytes. CD48 is probably involved in regulating T-cell activation.

 

前沿进展

 
 
货号/价格
文档
联系电话:
+86 400-682-2521(全国)
010-53681107(北京)
021-50850665(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
CD48靶点信息
英文全称:Cluster of differentiation 48
中文全称:分化簇48
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:0详情
最高研发阶段:终止
查看更多信息
前沿进展
点击查看详细

消息提示

请输入您的联系方式,再点击提交!

确定