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Human CD48 / SLAMF2 Protein, Mouse IgG2a Fc Tag, low endotoxin

分子别名(Synonym)

CD48,BCM1,SLAMF2,BLAST,BLAST1,MEM-102,TCT.1,BCM-1,SLAMF-2,BLAST-1

表达区间及表达系统(Source)

Human CD48, Mouse IgG2a Fc Tag, low endotoxin (BC1-H5258) is expressed from human 293 cells (HEK293). It contains AA Gln 27 - Ser 220 (Accession # P09326-1).

Predicted N-terminus: Gln 27

蛋白结构(Molecular Characterization)

Online(Gln 27 - Ser 220) P09326-1

This protein carries a mouse IgG2a Fc tag at the C-terminus.

The protein has a calculated MW of 49.2 kDa. The protein migrates as 64-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human CD48, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. BC1-H5258) SDS-PAGE gel

Human CD48, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human 2B4, Fc Tag (Cat. No. 2B4-H5255) at 2 μg/mL (100 μL/well) can bind Human CD48, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. BC1-H5258) with a linear range of 2-31 ng/mL (QC tested).

Protocol

Biotinylated Human SPR

Immobilized Biotinylated Human 2B4, Avitag,His Tag (recommended for biopanning) (Cat. No. 2B4-H82E9) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.2 μg/well) plate, can bind Human CD48, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. BC1-H5258) with a linear range of 0.3-5 ng/mL (Routinely tested).

Protocol

背景(Background)

CD48 antigen is also known as Signaling lymphocytic activation molecule 2, SLAM family member 2 (SLAMF2), Leukocyte antigen MEM-102, BCM1 surface antigen, B-lymphocyte activation marker BLAST-1. CD48 is a 65 kDa GPI-linked protein which belongs to the CD2 subfamily of immunoglobulin superfamily molecules. CD48 antigen contains two Ig-like C2-type (immunoglobulin-like) domains and two Ig-like V-type (immunoglobulin-like) domain. CD48 interacts with CD244 in an heterophilic manner. CD48 is ligand for CD2. CD48 might facilitate interaction between activated lymphocytes. CD48 is probably involved in regulating T-cell activation.

 

前沿进展

 
货号/价格

BC1-H5258-100ug / ¥3000

BC1-H5258-1mg (500ug X 2) / ¥19800

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CD48靶点信息
英文全称:Cluster of Differentiation 48
中文全称:分化簇48
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:2详情
最高研发阶段:临床一期
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