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Human Mesothelin / MSLN (296-580) Protein, His Tag, premium grade

分子别名(Synonym)

MSLN,Mesothelin,MPF

表达区间及表达系统(Source)

Human Mesothelin (296-580), His Tag, premium grade (MSN-H522a) is expressed from human 293 cells (HEK293). It contains AA Glu 296 - Gly 580 (Accession # AAH09272 ).

Predicted N-terminus: Glu 296

It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.

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蛋白结构(Molecular Characterization)

Mesothelin Structure

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 33.0 kDa. The protein migrates as 38-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.01 EU per μg by the LAL method.

无菌(Sterility)

The sterility testing was performed by membrane filtration method.

支原体(Mycoplasma)

Negative.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Mesothelin SDS-PAGE

Human Mesothelin (296-580), His Tag, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

Mesothelin SEC-MALS

The purity of Human Mesothelin (296-580), His Tag, premium grade (Cat. No. MSN-H522a) is more than 90% and the molecular weight of this protein is around 32-47 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-FACS

Mesothelin FACS

2e5 of Anti-MSLN CAR-293 cells were stained with 100 μL of 1 μg/mL of Human Mesothelin (296-580), His Tag, premium grade (Cat. No. MSN-H522a) and negative control protein respectively, washed and then followed by His Tag PE-conjugated Antibody and analyzed with FACS (QC tested).

Protocol

 

活性(Bioactivity)-ELISA

Mesothelin ELISA

Immobilized Human Mesothelin (296-580), His Tag, premium grade (Cat. No. MSN-H522a) at 0.5 μg/mL (100 μL/well) can bind Anti-MSLN MAb, Human IgG1 with a linear range of 0.2-3 ng/mL (QC tested).

Protocol

 

活性(Bioactivity)-BLI

Mesothelin BLI

Loaded Monoclonal Anti-Human MSLN Antibody, Human IgG1 on Protein A Biosensor, can bind Human Mesothelin (296-580), His Tag, premium grade (Cat. No. MSN-H522a) with an affinity constant of 3.57 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

Mesothelin (MSLN) is also known as CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, which belongs to the mesothelin family. Mesothelin / MSLN can be proteolytically cleaved into the following two chains by a furin-like convertase: Megakaryocyte-potentiating factor (MPF) and the cleaved form of mesothelin. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro.

 

前沿进展

 
 
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Mesothelin靶点信息
英文全称:Mesothelin
中文全称:间皮素
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:33详情
最高研发阶段:临床二期
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