分子别名（Synonym）
LAG3,CD223,FDC
表达区间及表达系统（Source）
Human LAG-3, His Tag, low endotoxin (LA3-H522a) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Leu 450 (Accession # P18627-1).
Predicted N-terminus: Leu 23
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 47.1 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 0.01 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human LAG-3, His Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
获取HPLC数据
活性（Bioactivity）-ELISA
Immobilized Anti-Human LAG3 MAb, Human IgG1 at 2 μg/mL (100 μL/well) can bind Human LAG-3, His Tag, low endotoxin (Cat. No. LA3-H522a) with a linear range of 1-6 ng/mL (QC tested).
Protocol
文献引用（Citations）
Antibodies binding lag-3 and uses thereof
Authors: X Kang, et al
Journal: US20190016800A1 2019
Application: ELISA & BLI
背景（Background）
Lymphocyte activation gene 3 protein (LAG3) is also known as CD antigen CD223 and protein FDC, w