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Human LAG-3 / CD223 Protein, His Tag, low endotoxin

分子别名(Synonym)

LAG3,CD223,FDC

表达区间及表达系统(Source)

Human LAG-3, His Tag, low endotoxin (LA3-H522a) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Leu 450 (Accession # P18627-1).

Predicted N-terminus: Leu 23

蛋白结构(Molecular Characterization)

Online(Leu 23 - Leu 450) P18627-1

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 47.1 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.01 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human LAG-3, His Tag, low endotoxin (Cat. No. LA3-H522a) SDS-PAGE gel

Human LAG-3, His Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

获取HPLC数据

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Anti-Human LAG3 MAb, Human IgG1 at 2 μg/mL (100 μL/well) can bind Human LAG-3, His Tag, low endotoxin (Cat. No. LA3-H522a) with a linear range of 1-6 ng/mL (QC tested).

Protocol

文献引用(Citations)

背景(Background)

Lymphocyte activation gene 3 protein (LAG3) is also known as CD antigen CD223 and protein FDC, w