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ELISA Assay Kit for Anti-HER-2 h-mAb in Human Serum

Cat. No. Components Size (480 tests)
PK401-213 Human HER-2 10 μg
PK402-213 Biotinylated anti-HER-2 antibody 10 μg
PK403-213 Streptavidin-HRP 10 μg
PK404-213 Anti-HER-2 antibody 30 μg

Background/背景

How a therapeutic antibody is metabolized in the body is pertinently relevant to its efficacy. Therefore, pharmacokinetics study is an important part of the drug development.

HER-2 is probably the most targeted molecule in today’s pharmaceutical industry, thanking to the clinical success of Herceptin®. Many investigational anti-HER-2 mAbs are being developed. There is a growing need for a standard assay that can be used to facilitate the study of their pharmacokinetics.

Assay Principles/原理

The enclosed ELISA assay kit for anti-HER-2 h-mAb in human serum is based on an enzyme immunoassay (ELISA) between recombinant HER-2 protein (ECD) and a biotinylated anti-HER-2 monoclonal antibody. The method employs the principle of competitive ELISA, allowing quantification of monoclonal anti-HER-2 antibodies in human serum.

The assay only involves the following steps:

1. Coat the plate with human HER-2;

2. Add the mixture of your sample and biotinylated anti-HER-2 antibody;

3. Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate;

4. Record the OD readings and analyze the serum concentration.

Reconstitution/重构方法

See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Advantages/产品优势

Wide Coverage

There is no requirement for the species and subtype of the antibody.

Easy Process

There is no longer a need to generate anti-drug antibodies for PK studies.

Clean Result

The use of the biotinylated antibody in the competitive ELISA significantly alleviates the issue of background noise issue that often occurs in traditional ELISA method.

Storage/存储

Upon receipt, please store all items at -20℃.

After reconstitution, the stock solution should be kept at -80℃.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. Room temperature (RT) for 1 month in lyophilized state;
  2. -20℃ for 6 months in lyophilized state;
  3. -80℃ for 4 months under sterile conditions after reconstitution.

Typical Data/典型数据

前沿进展

货号/价格

EHH-V1-480tests / ¥15110

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Her2靶点信息
英文全称:Receptor protein-tyrosine kinase erbB-2
中文全称:受体蛋白酪氨酸激酶 erbB-2
种类:Homo sapiens
上市药物数量:13详情
临床药物数量:96详情
最高研发阶段:批准上市
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