Cynomolgus CD3 epsilon, His Tag (CDE-C5226) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Asp 117 (Accession # Q95LI5-1).
Predicted N-terminus: Gln 22
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 12.2 kDa. The protein migrates as 10 kDa and 14-21 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Cynomolgus CD3 epsilon, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Mouse Anti-Human CD3 (SP34-2) at 2 μg/mL (100 μL/well) can bind Cynomolgus CD3 epsilon, His Tag (Cat. No. CDE-C5226) with a linear range of 0.05-1.6 ng/mL (QC tested).
SP34-2 (Mouse IgG1) captured on CM5 chip via anti-mouse antibodies surface, can bind Cynomolgus CD3E, His Tag (Cat. No. CDE-C5226) with an affinity constant of 0.981 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
CD3e molecule, epsilon is also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. CD3E gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2.