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Cynomolgus CD3 epsilon&CD3 delta Heterodimer Protein, His Tag&Flag Tag

分子别名(Synonym)

CD3E & CD3D,CD3 delta & CD3 epsilon,CD3 delta / epsilon

表达区间及表达系统(Source)

Cynomolgus CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag (CDD-C52W4) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Asp 117 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # Q95LI5 (CD3E) & Q95LI8 (CD3D)).

Predicted N-terminus: Gln 22 (CD3E) & Phe 22 (CD3D)

蛋白结构(Molecular Characterization)

Online(Gln 22 - Asp 117 (CD3E) & Phe 22 - Ala 105 (CD3D)) Q95LI5 (CD3E) & Q95LI8 (CD3D)

Cynomolgus CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag is produced by co-expression of CD3E and CD3D, has a calculated MW of 15.5 kDa (CD3E) and 14.5 kDa (CD3D). Subunit CD3E is fused with his tag at the C-terminus and subunit CD3D is fused with flag tag at the C-terminus. The predicted N-terminus is Gln 22 (CD3E) and Phe 22 (CD3D). The reducing (R) heterodimer protein migrates as 19-22 kDa and 25-40 kDa due to glyosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>92% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Cynomolgus CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag (Cat. No. CDD-C52W4) SDS-PAGE gel

Cynomolgus CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Mouse Anti-Human CD3 (SP34-2) at 1 μg/mL (100 μL/well) can bind Cynomolgus CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag (Cat. No. CDD-C52W4) with a linear range of 0.2-5 ng/mL (QC tested).

Protocol

背景(Background)

T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

 

前沿进展

 
货号/价格

CDD-C52W4-100ug / ¥3500

CDD-C52W4-1mg / ¥19800

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