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ELISA Assay Kit for Anti-PD-1 h-mAb in Monkey Serum

Cat. No. Components Size (96 tests) Size (480 tests)
PK301-213 Human PD-1 15 μg 60 μg
PK302-213 Biotinylated anti-PD-1 antibody 10 μg 10 μg
PK303-213 Streptavidin-HRP 5 μg 25 μg
PK304-213 Anti-PD-1 antibody 30 μg 30 μg

Background/背景

How a therapeutic antibody is metabolized in the body is pertinently relevant to its efficacy. Therefore, pharmacokinetics study is an important part of the drug development.

PD-1 is probably the most targeted molecule in today’s pharmaceutical industry, thanking to the clinical success of Keytruda® and Opdivo®. Many investigational anti-PD-1 mAbs are being developed. There is a growing need for a standard assay that can be used to facilitate the study of their pharmacokinetics.

Assay Principles/原理

The enclosed ELISA assay kit for anti-PD-1 h-mAb in monkey serum is based on an enzyme immunoassay (ELISA) between recombinant PD-1 protein (ECD) and a biotinylated anti-PD-1 monoclonal antibody. The method employs the principle of competitive ELISA, allowing quantification of monoclonal anti-PD-1 antibodies in monkey serum.

The assay only involves the following steps:

1. Coat the plate with human PD-1;

2. Add the mixture of your sample and biotinylated anti-PD-1 antibody;

3. Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate;

4. Record the OD readings and analyze the serum concentration.

Reconstitution/重构方法

See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Advantages/产品优势

Wide Coverage

This kit has been tested for multiple investigational PD-1 antibodies, as well as the commercially available Keytruda® and Opdivo®. There is no requirement for the species and subtype of the antibody.

Easy Process

There is no longer a need to generate anti-drug antibodies for PK studies.

Clean Result

The use of the biotinylated antibody in the competitive ELISA significantly alleviates the issue of background noise issue that often occurs in traditional ELISA method.

Storage/存储

Upon receipt, please store all items at -20℃.

After reconstitution, the stock solution should be kept at -80℃.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. Room temperature (RT) for 1 month in lyophilized state;
  2. -20℃ for 1 year in lyophilized state;
  3. -80℃ for 4 months under sterile conditions after reconstitution.

Typical Data/典型数据


Name Pembrolizumab Nivolumab Therapeutic PD-1 Antibody J, Human IgG4 Therapeutic PD-1 Antibody X, Human IgG4 Therapeutic PD-1 Antibody H, Human IgG4
Detection Range (μg/ml) 0.03125-20 0.03125-20 0.03125-20 0.03125-20 0.03125-20
Sensitivity (μg/ml) 0.15625 0.15625 0.15625 0.15625 0.15625
%Recovery 86-111 90-117 87-104 96-107 101-112

前沿进展

货号/价格

EPC-V1-96tests / ¥3270

EPC-V1-480tests / ¥11740

文档
联系电话:
400-682-2521(全国)
010-57737274(北京)
021-31604788(上海)
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
techsupport@acrobiosystems.com
PD-1靶点信息
英文全称:Programmed cell death protein 1
中文全称:细胞程序性死亡-1
种类:Homo sapiens
上市药物数量:2详情
临床药物数量:38详情
最高研发阶段:批准上市
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